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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 522-526
DOI: https://doi.org/10.20546/ijcmas.2020.909.066


Detection and Molecular Characterization of Peste Des Petits Ruminants Virus from Goat Flocks in Ambala, Haryana
Vandna Bhanot1*, Aman Kumar2 and Naresh Jindal3
1ADIO, Disease Investigation Laboratory, LUVAS, Ambala, India
2ABT, LUVAS, Hisar, India
3VPHE, LUVAS, Hisar, India
*Corresponding author
Abstract:

A Peste des petits ruminant (PPR) is a highly contagious viral disease of sheep and goats characterized by high morbidity and mortality. The present study was conducted to determine comparative efficacy of F and N gene based reverse transcription polymerase chain reaction for molecular detection of PPR virus from clinical samples from goat flocks in Ambala district of Haryana. Out of 36 clinical samples tested, 28 samples were found positive by N gene-based primers, however, 23 samples were found positive by F gene based primer set. Hence, N gene-based RT-PCR used in this study was found to be more sensitive for detection of PPRV as compared to F gene based assay. The nucleotide sequence analysis and inferred phylogenetic relationship revealed the presence of lineage IV of PPRV.


Keywords: Goat Flocks,Peste des petits ruminant (PPR)
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How to cite this article:

Vandna Bhanot, Aman Kumar and Naresh Jindal. 2020. Detection and Molecular Characterization of Peste Des Petits Ruminants Virus from Goat Flocks in Ambala, Haryana.Int.J.Curr.Microbiol.App.Sci. 9(9): 522-526. doi: https://doi.org/10.20546/ijcmas.2020.909.066