The aim of this study was to evaluate the effect of the hormonal treatment on uterine involution in dystocia affected buffaloes. The experiment was carried out with total 33 buffaloes, out of which 27 were dystocia affected buffaloes presented at University clinic, GADVASU for the treatment which were further divided into 2 groups on the basis of treatment with prostaglandin and gonadotropin releasing hormone analogue (Group I: n=18, PGF2α on day 7 and GnRH on day 14 postpartum) or not (Group II: n=9, No treatment).Six normal calving buffaloes served as control group with no hormonal treatment (Group III). Rectal examination was done on day 1 (day of parturition), 7, 14 and 35 postpartum to assess various parameters. The present study demonstrated significant (P<0.05) involution of uterus in hormonal treated dystocia affected and normally calved buffaloes (Group I and III) as compared to non-treated buffaloes (Group II). On day 1 and 7uterine horns remained in the abdominal cavity and size of the uterine horn remained greater than the size of the cervix in all the three groups. On day 14, 44.4 and 55.6 % animals had their uterine horns located between pelvic and abdominal cavities in group I and II, respectively, whereas in group III uterine horns were in the abdominal cavity. On day 35, in group I and II 16.7 vs 83.3 and 55.6 vs 44.4 % dystocia affected buffaloes had location of uterine horns in between pelvic and abdominal cavity vs pelvic cavity, respectively, whereas uterine horns were located within the pelvic cavity in group III. On day 14, in group I and III, uterine size became equal to the size of the cervix in 22.2 and 33.3 % animals, respectively, whereas in group II 100 % animals had uterine horn size greater than cervix (P<0.05). On day 35, size of the uterine horn in 72.2, 44.4 and 100.0 % of animals was smaller than cervix in group I, II and III, respectively. On day 1, 7, 14 and 35, no significant difference was observed in uterine tonicity of dystocia affected and normal calving buffaloes. In all the groups, on day 14 and 35 a mild change in uterine tonicity from low to moderate was observed. In conclusion, administration of prostaglandin and gonadotropin releasing hormone may help to hasten uterine involution and subsequent ovarian activity in dystocia affected buffaloes.

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