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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 211-221
DOI: https://doi.org/10.20546/ijcmas.2020.909.027


Morphological Characterization of Kurnool Strains of Chickpea Collar Rot Casual Agent Sclerotium rolfsii Sacc.
Saileela1, M. Lal Ahamed1, J. V. Ramana1* and S. Khayum Ahamed2
1Department of Molecular Biology and Biotechnology, APGC, Lam, Guntur, India
2Department of Plant Pathology, AICRP on Chickpea, RARS, Nandyal, India
*Corresponding author
Abstract:

Sclerotium rolfsii causing collar rot is a major disease affecting crop stand and yield in chickpea. A comprehensive study was performed to report the variability in Sclerotium rolfsii isolates present in a major chick pea growing area, Kurnool District of Andhra Pradesh. A roving survey was conducted to collect 50 samples and was used for isolation of disease causing pathogen. 18 isolates of Sclerotium rolfsii were isolated to study the variability in morphological and cultural characteristics in the present study. The variation noted for mycelial traits and sclerotial characters were very huge. The trait, total colony diameter, values after 72 hours of incubation ranged from 42 mm to 72 mm and was exploited to classify the isolates as slow, medium and fast. The colour of the mycelia colony was pure white to dull white while, colony morphology or topography varied from compact to fluffy in some isolates. Further, the trait, sclerotia initiation, varied significantly from 5 to 10 days indicating the aggressiveness of the isolates in causing the disease. Sclerotia colour was light brown to dark brown and black in KCSR 16 and the shape was round, spherical to oval and irregular in few. The test weight (100 sclerotia) was ranged from 40 to 320mg and the number varied from 25 to 124 indicating presence of sufficient variability among the isolates for characterization. This characterization of S.rolfsii isolates is necessary to devise effective management strategies to control the disease.


Keywords: Sclerotium rolfsii, Morphological characterization, Mycelia and Sclerotial parameters
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How to cite this article:

Saileela, M., M. Lal Ahamed, J. V. Ramana and Khayum Ahamed, S. 2020. Morphological Characterization of Kurnool Strains of Chickpea Collar Rot Casual Agent Sclerotium rolfsii Sacc..Int.J.Curr.Microbiol.App.Sci. 9(9): 211-221. doi: https://doi.org/10.20546/ijcmas.2020.909.027