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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 14-21
DOI: https://doi.org/10.20546/ijcmas.2020.909.002


Molecular Characterization and Serogroup Prevalence of Dichelobacter nodosus from the Cases of Ovine Footrot in Andhra Pradesh and Telangana States of India
Vijayalakshmi1*, D. Sreenivasulu2, N. Vinodkumar3, Raniprameela4 and A. Karthik4
1LRS, Palamaneru, SVVU, India
2SVVU, Tirupati, India
3Department of Microbiology, CVSc, Tirupati, India
4SLDL, SVVU, Tirupati, India
*Corresponding author
Abstract:

A total of 338 foot swabs were collected from different districts of AP and Telangana state and were subjected to PCR targeting 16S rRNA gene of
D. nodosus. Out of 338 samples, 86 samples were found to be positive for PCR suggesting the presence of D. nodosus. Further all the PCR positive samples were subjected to multiplex-PCR for serogrouping. Out of 86 positive samples, 53 found to be belonged to ‘B’ serogroup followed by 24 ‘I’ serogroup, 6 ‘A’ serogroup, 2 ‘E’ serogroup and one sample ‘H’ serogroup. The overall prevalence of the D. nodosus was calculated as 16%. The prevalence of D. nodosus serogroups ‘B’, ‘I’, ‘A’, ‘E’ and ‘H’ was 61.6%, 27.9%, 6.9%, 2.3% and 1.2% respectively.


Keywords: Dichelobacter nodosus,Ovine Footrot
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How to cite this article:

Vijayalakshmi, S., D. Sreenivasulu, N. Vinodkumar, D. Raniprameela and Karthik, A. 2020. Molecular Characterization and Serogroup Prevalence of Dichelobacter nodosus from the Cases of Ovine Footrot in Andhra Pradesh and Telangana States of India.Int.J.Curr.Microbiol.App.Sci. 9(9): 14-21. doi: https://doi.org/10.20546/ijcmas.2020.909.002