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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 3971-3978
DOI: https://doi.org/10.20546/ijcmas.2020.908.457


Protein Profiling of Resistant and Susceptible Mutant Lines of Rice Variety Swarna in Response to Rhizoctonia Solani AG1 IA Infection
Reeshu Singh1,  Sumant  Pratap Singh1 , Ankit Singh2, Prabhat Kumar Singh1, D.K. Dwivedi1 and N.A. Khan1
1Department of Plant Molecular Biology and Genetic Engineering, A.N. D. University of             Agriculture and Technology, Kumarganj Ayodhya, -224229 (UP).
2Department of Crop Physiology, A.N. D. University of Agriculture and Technology,    Kumarganj Ayodhya, -224229 (UP), India
*Corresponding author
Abstract:

Rice sheath blight, caused by Rhizoctonia solani, is one of the most devastating diseases for stable rice production in most rice-growing regions of the world. Currently, studies of the molecular mechanism of rice sheath blight resistance are scarce. Here, we used mutated rice population of the variety swarna induced by the sodium azide (NaN3). The variability in disease reaction was observed among mutated rice lines. Out of the total 1000 mutant plants, 47 plants were screened that show low disease index, ranged between 0 to 5 were selected for next  generation. In M1 generation the selected genotypes show differences for disease index, only 12 lines shows resistance in M 1 generation in field condition and in humidity chamber condition only 8 lines shows resistance. The protein analysis by SDS-PAGE showed that the resistance lines have one extra band in between 33 kDa and 43 kDa that is not showing in moderate and susceptible lines. Thus, these resistance lines could be considered a potential source for disease resistance against the sheath blight of rice and could be used further in the crossing programme for development of sheath blight resistant rice variety.


Keywords: Mutation, Disease screening, Sheath blight, Resistance, Protein profiling
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How to cite this article:

Reeshu Singh, Sumant Pratap Singh, Ankit Singh, Prabhat Kumar Singh, D.K. Dwivedi and Khan, N.A. 2020. Protein Profiling of Resistant and Susceptible Mutant Lines of Rice Variety Swarna in Response to Rhizoctonia Solani AG1 IA Infection.Int.J.Curr.Microbiol.App.Sci. 9(8): 3971-3978. doi: https://doi.org/10.20546/ijcmas.2020.908.457