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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 3964-3970
DOI: https://doi.org/10.20546/ijcmas.2020.908.456


Analysis of Leaf and Seed Protein of Pigeonpea Genotype (Cajanus cajan L. Mills Paugh) Including One Wild Species Revealed by Gel Electrophoresis
Bavita Yadav*, N. A. Khan, Tanvi Chauhan, Pratibha Yadav and D. K. Dwivedi
Department of PMB & GE, A. N. D. University of Agriculture & Technology,
Kumarganj, Ayodhya (224229), India
*Corresponding author
Abstract:

Pigeonpea is the major source of dietary protein for vegetarian population in India and other developing countries. The following study was taken to understand the genetic relationship between genotypes on the basis of protein profiling of leaves and seed protein. Ten genotypes of Pigeon pea [Cajanus cajan (L.) Millspaugh] including one wild species was sown in the field at Student Instructional Farm, ANDUA & T Kumarganj Ayodhya. Total protein was extracted using 0.1 M Sodium phosphate buffer (pH 7.2) containing NaH2PO4 and Na2HPO4. Protein was electrophoresed on 12% SDS- PAGE along with standard protein marker and detected by commassive brilliant blue staining. Pigeonpea leaves and seeds protein showed variability in banding pattern of polypeptide on gel. RM values of different pigeonpea genotype were measured. The results showed that both leaves and seeds protein migrated between a range of < 10 to 250 kDa. NDA-3, MAL-6 and MAL-13 had minimum protein bands (8 bands) while maximum protein bands was observed in NDA-14-6 (12 bands) in leaves. In protein profile of seeds it has been seen that Cajanus scarabaeoides showed maximum bands (20 bands) while NDA-2 showed minimum protein band (11). All the accessions commonly showed at least six major bands in leaves while 11 major bands in seeds. In this context we can say that seeds of wild genotype contain more protein than cultivated pigeonpea genotype. The main objective of this study is to reveal intra-specific similarities and genetic diversity in protein content amongst ten genotypes of pigeon pea.


Keywords: Pigeonpea, Cajanus scarabaeoides, SDS-PAGE
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How to cite this article:

Bavita Yadav, N. A. Khan, Tanvi Chauhan, Pratibha Yadav and Dwivedi. D. K. 2020. Analysis of Leaf and Seed Protein of Pigeonpea Genotype (Cajanus cajan L. Mills Paugh) Including One Wild Species Revealed by Gel Electrophoresis.Int.J.Curr.Microbiol.App.Sci. 9(8): 3964-3970. doi: https://doi.org/10.20546/ijcmas.2020.908.456