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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 3955-3963
DOI: https://doi.org/10.20546/ijcmas.2020.908.455


Effect of Duration of Cold Pretreatment and Plant Growth Regulators on Callusing and Green Plant Regeneration of Indica (Oryza sativa L.) Rice
Muntazir Mushtaq1*, Anil Kumar Singh1, Romesh Kumar Salgotra1,Manmohan Sharma1, R. K. Samnotra2, Vikas Sharma3, Sunali Mahajan4,Iqbal Jeelani Bhat4 and Rizwan Yousuf4
1School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, J&K, 180009, India
2Division of Vegetable Science, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, J&K, 180009, India
3Division of Biochemistry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, J&K, 180009, India
4Division of Statistics and Computer Science, SKUAST Science, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, J&K, 180009, India
*Corresponding author
Abstract:

Improvement of indica rice lines is a challenging task through application of anther culture as they are recalcitrant to culture distinctive to japonica rices. Current investigation aimed to verify the effect of cold pretreatment and phytohormones on the anther culture response of 6 crosses. Cold pretreatment for 7-10 days at 100C was found to have profound effect on callus induction frequency irrespective of the media employed and the prolonged treatment over the optimum showed to be inhibitory. Of the different auxins (2,4-D, NAA) and cytokinins (Kinetin, BAP)and their combinations studied, a ratio of 1:4 for 2,4-D and NAA and 1:3:1 ratio of Kinetin: BAP: NAA ratio proved to be optimal for callus induction and green plant regeneration respectively. This information suggests the opportunity of exploiting hybrid vigor in indica rice cultivars for the development of doubled haploids with more yield and yield attributes from elite hybrid crosses.


Keywords: Anther culture, In vitro, Cold pretreatment, Plant growth regulators, Indica rice, Medium
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How to cite this article:

Muntazir Mushtaq, Anil Kumar Singh, Romesh Kumar Salgotra, Manmohan Sharma, R. K. Samnotra, Vikas Sharma, Sunali Mahajan, M. Iqbal Jeelani Bhat and Rizwan Yousuf. 2020. Effect of Duration of Cold Pretreatment and Plant Growth Regulators on Callusing and Green Plant Regeneration of Indica (Oryza sativa L.) Rice.Int.J.Curr.Microbiol.App.Sci. 9(8): 3955-3963. doi: https://doi.org/10.20546/ijcmas.2020.908.455