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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 3904-3914
DOI: https://doi.org/10.20546/ijcmas.2020.908.449


Linkage Mapping and Identification of QTLS Responsible for Earliness in Bread Wheat (Triticum aestivum L.) in F2:3 Mapping Population
A. Delvadiya1*, D. R. Mehta2, A. K. Nandha1 and R. R. Rathod1
1Department of Biotechnology, 2Department of Genetics and Plant Breeding, Junagadh Agriculture University, Junagadh-362001, India
*Corresponding author
Abstract:

Earliness, an adaptive trait and factor of variation for agronomic characters, is a major trait in plant breeding. In present investigation, the experimental material comprised of P1, P2, F1, F2 and F2:3 generations of wheat crossDL-788-2 X GW-322 for earliness related traits with objective of linkage and QTL mapping in bread wheat. Out of 200 SSR markers screened for parental polymorphism for earliness related traits, only 11% of SSR markers showed good polymorphism between two parental lines. Out of 22 tests, all the test markers showed non-significant chi-square which revealed that observed data were agreement with expected ratio of 1:2:1 segregation ratio. The linkage map was constructed using software Ici Mapping v.4.1 and recombination frequencies were converted into map distance using Kosambi’s mapping function. The markers were grouped with minimum logarithm of the odds (LOD) of 3.0 with walking speed was set at 1.0 cM. Four linkage groups with a total map length of 267.12 cM were constructed using data from 22 marker loci for 74 F2 plants that ranged from minimum of 8.62 cM (LG4) to maximum of 126.56 cM (LG1).Genotypic data of F2 and phenotypic data of on 74 F2:3 lines were analyzed for identification of the main effect QTLs using the software ICIM-ADD mapping in QTL IciMappingV4.1. A linkage map of earliness related traits output data file was used for the construction of QTL mapping. One QTL was identified for days to 50% flowering (LG1 at 58.0 cM, LOD 3.06, 18 PVE %) and two QTLs for days to maturity (LG1 at 21 cM, LOD 8.89, 31.51 PVE% and LG3 at 38 cM, LOD 12.83, 45.16 PVE%).with use of molecular marker and QTL mapping complex from of earliness traits and their underlying genes are now far more accessible which can be routinely used by breeders in marker assisted selection in wheat breeding programs.


Keywords: Linkage mapping, QTL mapping, SSR marker, Bread wheat
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How to cite this article:

Delvadiya, N. A., D. R. Mehta, A. K. Nandha and Rathod, R. R. 2020. Linkage Mapping and Identification of QTLS Responsible for Earliness in Bread Wheat (Triticum aestivum L.) in F2:3 Mapping Population.Int.J.Curr.Microbiol.App.Sci. 9(8): 3904-3914. doi: https://doi.org/10.20546/ijcmas.2020.908.449