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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 3857-3865
DOI: https://doi.org/10.20546/ijcmas.2020.908.444


Phenotypic and Genotypic Patterns of Beta-Lactam Resistance among Escherichia coli Clinical Isolates
Ghada Hani Ali*
Department of Microbiology and immunology, Faculty of pharmacy and Drug Manufacturing, Pharos University, Alexandria, Egypt
*Corresponding author
Abstract:

The aim of the present study was to determine the phenotypic resistance pattern to β-lactam antibiotics among Escherichia coli clinical isolates and to identify the genetic determinants responsible for β-lactam antibiotic resistance. Forty Escherichia coli isolates were included in this study. Identification of tested strains was confirmed using MALDI-TOF/MS. Phenotypic and genotypic β-lactamase patterns were investigated. Twelve isolates were resistant to carbapenems, while most of them exhibited resistance to the one or more of the third and fourth generation cephalosporins (ESC) (35 out of 40). Phenotypically, the production of extended-spectrum beta-lactamase (ESBL), metallo-β-lactamases (MBL), and carbapenemases was detected in 31, 8, and 15 isolates, respectively. Genotypically, ESBL and carbapenemase encoding genes were detected in 34 and 19 isolates, respectively. The results of the current study indicate the high prevalence of β-lactam resistance among Escherichia coli isolates.


Keywords: ESBL, Carbapenemase, MBL, E. coli,PCR, MALDI-TOF
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How to cite this article:

Ghada Hani Ali 2020. Phenotypic and Genotypic Patterns of Beta-Lactam Resistance among Escherichia coli Clinical Isolates.Int.J.Curr.Microbiol.App.Sci. 9(8): 3857-3865. doi: https://doi.org/10.20546/ijcmas.2020.908.444