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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(3): 47-56
DOI: http://dx.doi.org/10.20546/ijcmas.2016.503.007


Phenotypic Detection of Biofilms in Candida Species Isolated from Various Clinical Samples
M. Bansal*, S.A. Samant, S. Singh and A. Talukdar
Department of Microbiology, MGM Medical College and Hospital, Kamothe, Navi Mumbai, India
*Corresponding author
Abstract:

Since biofilms have been considered as a virulence factor contributing to the Candidal infection, a reliable method for their diagnosis is necessary. In this study, we screened 100 clinical isolates of Candida spp. by Congo Red Agar Method, Test Tube method and Micro titer Plate Method. Biofilms have great significance in public health, because biofilm-associated Candida exhibit dramatically decreased susceptibility to antimicrobial agents. This susceptibility may be intrinsic (as a natural outcome of growth in the biofilm) or acquired (due to transfer of extra chromosomal elements to susceptible organisms in the biofilm). It is likely that biofilms evade anti microbial challenges by multiple mechanisms. In this study 57% of the clinical Candida isolates showed Biofilm production by Test tube method. It was found that C. albicans had a little lower percentage of biofilm positivity (4/9 = 44.4%) as compared to non-albicans Candida spp. (53/91 = 58.2%). Antifungal efficacy of Coconut oil and Eucalyptus oil was also tested in this study against all Candida isolates. Eucalyptus oil was observed to be a better antifungal agent than Coconut oil in the present study. When coconut oil was tested against all Candida albicans isolates, the sensitivity of biofilm non producers was higher in comparison to biofilm producers.


Keywords: Candida biofilm, Phenotypic detection, Antifungal, Plant oils.
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How to cite this article:

Bansal, M. S.A., Samant, S. Singh and Talukdar, A. 2016.  Phenotypic Detection of Biofilms in Candida Species Isolated from Various Clinical Samples.Int.J.Curr.Microbiol.App.Sci. 5(3): 47-56. doi: http://dx.doi.org/10.20546/ijcmas.2016.503.007