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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 3789-3798
DOI: https://doi.org/10.20546/ijcmas.2020.908.437


Development and Evaluation of Synthetic Antigenic Peptides for Diagnosis of Infectious Bursal Disease
Upadhyay1*, V. Umapathi2 and B.K. Sarkar3
1AICRP on PHET, Department of Livestock Products Technology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India
2Department of Veterinary Physiology and Biochemistry, GB Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India
3Department of LPT, College of Veterinary Sciences and Animal Husbandry, R K Nagar, Tripura, India
*Corresponding author
Abstract:

Infectious Bursal Disease (IBD) is an economically important and contagious viral disease of poultry. An attempt was made for development of a synthetic peptide based diagnostic reagent. Cell culture adapted viral antigen was prepared by successful passage on CEF and then ultracentrifugation to get partially purified virus. Confirmation of the virus in was done by RT- PCR. The antigen was then used for the production of hyper immune sera in rabbits. Sera samples were also collected from known IBD vaccinated and non-vaccinated birds. Four different sequences were selected based on antigenic determinant analysis using sequence data available on Gene bank. Four MAPs, M1, M2, M3 and M4 were synthesized based on these sequences using FMOC chemistry. These peptides were then screened for their reactivity with the hyperimmune sera using indirect ELISA. Although all the peptides showed positive reaction, peptide M3 showed the highest reactivity. Screening was also done with the randomly selected 33 known bird sera for their reactivity pattern. It was found that all the peptides consistently differentiated positive and negative sera samples accordingly whether the samples were obtained from vaccinated or unvaccinated birds. This study offers a technology on designing of IBD virus specific synthetic peptides as diagnostic reagents for their use in IBD diagnosis.


Keywords: Synthetic peptide, IBDV, ELISA, Hyperimmune, Serum
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How to cite this article:

Upadhyay, S., V. Umapathi and Sarkar, B.K. 2020. Development and Evaluation of Synthetic Antigenic Peptides for Diagnosis of Infectious Bursal Disease.Int.J.Curr.Microbiol.App.Sci. 9(8): 3789-3798. doi: https://doi.org/10.20546/ijcmas.2020.908.437