Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 3506-3512
DOI: https://doi.org/10.20546/ijcmas.2020.908.405


Rapid and Sensitive Faecal based PCR Assays for the Detection of Parvoviruses in Laboratory Rats
M.R. Srinivasan1, K. Vijay2*, S. Ramesh1, A.K. Karuppannan2 and Y. Krishnamohan Reddy2
1Department of Veterinary Pharmacology and Toxicology, Madras Veterinary College, Vepery, Chennai-600007, India
2Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 051, India
*Corresponding author
Abstract:

Health status of laboratory rats indicates its suitability for the researches. They are prone to develop viral infections of subclinical nature caused by parvoviruses, which affects the research results adversely; Hence Implementation of health monitoring protocol is essential at the level of breeding colony itself. This study was intended to develop rapid and sensitive fecal-PCR assays to detect infections of multiple species of Parvoviruses affecting rats namely, Rat Minute Virus, Toolan’s Parvo Virus, Rat Parvo Virus and Kilham’s Rat Virus as an alternate approach to serology based health monitoring in a lab animal breeding unit. All the primers detected only in the presence of the respective templates. PCR assays of RMV and TPV consistently amplified as little as 40 fg and that of RPV and KRV consistently amplified as little as 4 fg of plasmid DNA. Specificity and sensitivity assays indicate that the PCR assays may be useful as diagnostic tools for rapid detection of natural acute viral infections. Further analysis of the primers in the positive laboratory animal colony is essential.


Keywords: Parvovirus, Fecal PCR assay, Health monitoring
Download this article as Download

How to cite this article:

Srinivasan, M.R., K. Vijay, S. Ramesh, A.K. Karuppannan and Krishnamohan Reddy, Y. 2020. Rapid and Sensitive Fecal based PCR Assays for the Detection of Parvoviruses in Laboratory Rats.Int.J.Curr.Microbiol.App.Sci. 9(8): 3506-3512. doi: https://doi.org/10.20546/ijcmas.2020.908.405