National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Heat shock proteins (Hsps) are equally well termed stress proteins, and their expression is termed the stress response. Assessing the heat shock protein at different temperature of 35, 40, 45 and 50º at pH 5.5, the four numbers of isolates were taken from each host species of Rhizobium (COS1, COG15, CO5, TNAU 14 and CRR6) and specific temperature of 35, 40, 45 and 50ºC respectively and compared with the control (reference culture maintained at 28ºC). Totally 20 number of isolates were taken and subjected for protein studies At 50°C, the protein content (0.92 mg ml-1 of cells) was higher but the strain CRR 6 and TNAU 14 had lower protein content of 0.72 and 0.81 mg ml-1 of cells respectively. Qualitative and quantitative differences in polypeptide patterns of rhizobial strains were detected after growth at 35, 40, 45 and 50°C when compared to the control (28°C) conditions. Mostly all the 20 temperature and acid tolerant rhizobial isolates revealed the synthesis of heat shock proteins at higher temperature. For example, rhizobial strains CO 5 and COG 15 revealed that the simultaneous overproduction of three polypeptides (60 / 36 / 43 kDa) when submitted to 35, 40 and 45°C but at 50°C only one polypeptide of molecular weight 60 kDa expressed.
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