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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 3198-3211
DOI: https://doi.org/10.20546/ijcmas.2020.908.365


Genetic Diversity Analysis of Table Potato Genotypes Using SSR Markers
R. S. S. Singh1*, A. Mishra1, M. K. Kar2, R. P. Sah2, M. Behera2 and A. Bal2
1All India Coordinated Research Project on Potato, Odisha University of Agriculture & Technology, Bhubaneswar, Odisha, India
2ICAR-National Rice Research Institute, Cuttack, Odisha, India
*Corresponding author
Abstract:

An experiment was carried out at AICRP on Potato under Odisha University of Agriculture and Technology, Bhubaneswar to study diversity among morphologically distinguishable potato genotypes with SSR markers. Twenty potato genotypes were laid out in a Randomized Block Design with four replications. Eight morphological characters were observed based on DUS characteristics to visually differentiate genotypes. In addition, fifteen SSR markers were used to depict clear-cut variation among genotypes with the aid of polymorphic bands. A total of 51 loci were amplified by 15 SSR markers which exhibited 92.16 percent polymorphism. The PIC values varied widely among 15 SSR loci tested and ranged from a minimum 0.2078 (STM1106) to maximum 0.7756 (STM0019) with an average of 0.5200. Heterozygosity values ranged from minimum 0.2659 (STM1106) to maximum 0.8047 (STM0019). The Jaccard’s dissimilarity coefficient was found to vary from 0.321 to 0.628. The binary data were used to calculate genetic dissimilarities based on Jaccard’s coefficient and UPGMA (Unweighted Pair Group Method using Arithmetical Means). A dendrogram was constructed using DARwin version 6 software, exhibiting pictorial expression of diverse genotypes. UPGMA divided the populations of 20 genotypes into six clusters; Cluster IV being the largest containing seven genotypes. Kufri Lima was found to be the most divergent from rest of the genotypes.


Keywords: Table potato, Diversity, Morphological characters, UPGMA, SSR markers
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How to cite this article:

Singh, B. R. S. S., A. Mishra, M. K. Kar, R. P. Sah, M. Behera and Bal, A. 2020. Genetic Diversity Analysis of Table Potato Genotypes Using SSR Markers.Int.J.Curr.Microbiol.App.Sci. 9(8): 3198-3211. doi: https://doi.org/10.20546/ijcmas.2020.908.365