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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 3161-3173
DOI: https://doi.org/10.20546/ijcmas.2020.908.360


Studies on Sensitivity of Blackgram (Vigna mungo (L.) Hepper) Varieties to Physical and Chemical Mutagens under in-vitro Condition
S. Devi1*, D. M. Reddy2, K. H. P. Reddy1, V. L. N. Reddy1,L. Reddy1 and P. Sudhakar3
1Department of Genetics and Plant Breeding, S. V. Agricultural College,
ANGRAU, Tirupati-517 502, India
2Regional Agricultural Research Station, ANGRAU, Tirupati, India
3ANGRAU, Lam, Guntur, India
*Corresponding author
Abstract:

The present investigation was taken up to study the sensitivity of two blackgram varieties viz., LBG 752 and TBG 104 to three mutagens on biological parameters and to determine the lethal dose (LD50) of mutagens. The seeds were treated with different doses/concentrations of gamma rays (200 Gy, 300 Gy, 400 Gy, 500 Gy and 600 Gy), EMS (Ethyl methane sulphonate) (0.2%, 0.3%, 0.4%, 0.5% and 0.6%) and MH (Maleic Hydrazide) (0.01%, 0.02% and 0.03%). MH treatment resulted in drastic reduction in seed germination percentage, shoot length and root length followed by EMS treatment and gamma rays treatment in both the varieties. The reduction in these characters with increasing dose/concentration of mutagens was more prominent in LBG 752 than TBG 104 indicating more sensitive response of LBG 752 to mutagen treatment than TBG 104.  LD50 values were estimated based on seed germination percentage and the values were 500.16 Gy for gamma rays, 0.396% for EMS and 0.016% for MH in LBG 752 and 552.92 Gy  for gamma rays, 0.554% for EMS and 0.028% for MH in TBG 104. These findings would greatly help for cost effective selection of variety and mutagens for successful generation of variation in mutation breeding programmes aimed at blackgram crop improvement.


Keywords: Blackgram, Mutagen, Probit analysis, Gamma rays, EMS, MH and LD50 value
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How to cite this article:

Devi, M. S., D. M. Reddy, K. H. P. Reddy, V. L. N. Reddy, D. L. Reddy and Sudhakar, P. 2020. Studies on Sensitivity of Blackgram (Vigna mungo (L.) Hepper) Varieties to Physical and Chemical Mutagens under in-vitro Condition.Int.J.Curr.Microbiol.App.Sci. 9(8): 3161-3173. doi: https://doi.org/10.20546/ijcmas.2020.908.360