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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 2930-2937
DOI: https://doi.org/10.20546/ijcmas.2020.908.328


Establishment of Efficient In-vitro Regeneration Protocol in ‘Malbhog’ Banana (Musa paradisiaca) using MWCNTs and Plant Growth Regulators
Indrani Chetia1, Amrita Kashyap Chaliha1, Manab Bikash Gogoi2* and Gaurav Verma3
1Dibrugarh University, Dibrugarh, Assam, India
2Department of Agricultural Biotechnology, Assam Agriculture University,
Jorhat, Assam, India
3Dr. S. S. Bhatnagar University Institute of Chemical Engineering & Technology, Panjab University, Chandigarh & Co-Coordinator, Centre for Nanoscience & Nanotechnology (U.I.E.A.S.T.), Panjab University
*Corresponding author
Abstract:

The present study was performed to establish an efficient and standard protocol for in vitro propagation of ‘Malbhog’ (Musa paradisiaca, AAB genome) banana under suitable culture conditions and to study the effect of growth hormones BAP & NAA on the growth of explants. Further, for the first time we report here the effect of MWCNTs (Multi walled carbon nano tubes) like FCNTs (Functionalized carbon nano tubes), D1 and D2 in ‘Malbhog’ In-vitro regeneration. It was also observed that TDZ at 0.11 mg/L showed the highest response (7.5 shoots per explants) in terms of shoot proliferation where as root induction was found to be highest in the MS media supplemented with 1 mg/L NAA. Also, the explants grown in MS Media with FCNTs at100 µg/mL (8.5 shoots per explants) exhibited acceleration of shoot proliferation in comparison to control without FCNTs.


Keywords: Multi walled carbon nano tubes, Functionalized carbon nano tubes, In-vitro regeneration of Malbhog banana
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How to cite this article:

Indrani Chetia, Amrita Kashyap Chaliha, Manab Bikash Gogoi and Gaurav Verma. 2020. Establishment of Efficient In-vitro Regeneration Protocol in ‘Malbhog’ Banana (Musa paradisiaca) using MWCNTs and Plant Growth Regulators.Int.J.Curr.Microbiol.App.Sci. 9(8): 2930-2937. doi: https://doi.org/10.20546/ijcmas.2020.908.328