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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 2442-2449
DOI: https://doi.org/10.20546/ijcmas.2020.908.279


RAPD Analysis for Genetic Diversity and Verification of Hybridity in Cowpea [Vigna unguiculata (L.) Walp.]
U. B. Pethe1*, N. S. Dodiya2, S. G. Bhave3 and S. V. Sawardekar4
1Department of Plant Breeding and Genetics, Rajasthan College of Agriculture, Udaipur, India
2Department of PB&G, RCA, Udaipur-313001, India
3Director of Extension Education, Dr B.S. Konkan Krishi Vidyapeeth, Dapoli (M.S.), India
4Dr B.S. Konkan Krishi Vidyapeeth, Dapoli (M.S.), India
*Corresponding author
Abstract:

Cowpea (Vigna unguiculata) is a legume crop growing in almost all parts of world including tropics and subtropics. This study was thus undertaken to assess genetic diversity among the different parents used for hybridization and confirmation of hybrids of cowpea (Vigna unguiculata) by using randomly amplified polymorphic DNA (RAPD) markers. RAPD profiles for 11 genotypes were generated with 20 random decamer primers. Out of 20 primers screened 15 primers gave scorable DNA fragments and each of the 15 primers revealed various levels of polymorphism. These primers generated 476 DNA fragments in the average range of 167bp to 3300bp, of which 367 were polymorphic. The percentage of polymorphism ranged from 35.29 to 100% with an average of 79.96%. The overall range of similarity among 11 genotypes was found to be very wide, ranging from 0.328 to 0.613 which indicates there was high variability among the cowpea genotypes under study. The RAPD primer OPA-18 was effectively used for confirmation of hybridity.


Keywords: Cowpea, Genetic diversity, RAPD markers, Hybridity
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How to cite this article:

Pethe, U. B., N. S. Dodiya, S. G. Bhave and Sawardekar, S. V. 2020. RAPD Analysis for Genetic Diversity and Verification of Hybridity in Cowpea [Vigna unguiculata (L.) Walp.].Int.J.Curr.Microbiol.App.Sci. 9(8): 2442-2449. doi: https://doi.org/10.20546/ijcmas.2020.908.279