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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 2337-2347
DOI: https://doi.org/10.20546/ijcmas.2020.908.268


Effect of Altrenogest Feeding in Prepubertal Buffalo Heifers Vis-A-Vis Plasma Estrogen-Progesterone Levels and Biochemical Profile
A. S. Aulakh1*, Prahlad Singh1, Gurpreet Singh2, S. S. Dhindsa1, Honparkhe1 and Arashdeep Kaur1
1Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
2Department of Veterinary Physiology and Biochemistry, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
*Corresponding author
Abstract:

A comparative study to evaluate the effect of altrenogest feeding on blood metabolic and hormonal profiling of prepubertal buffalo heifers, (n=20), 18-24 months of age and 250-300 kg body weight. Buffalo heifers were divided into three groups; Group I: (n=7) was fed oral Altrenogest (a progestin analogue, Regumate®, MSD, India, Ltd) at the rate 0.044 mg/kg body weight per day for 13 days (start day 1), GnRH analogue (20µg buserelin-acetate) IM on day 7, PGF2α analogue (500 µg cloprostenol sodium) IM on day 13 and GnRH analogue (20µg buserelin-acetate) IM on day 14 (modified ovsynch protocol),followed by FTAI on day15; Group II: (n=7) administered modified ovsynch protocol, followed by FTAI on day15; Group III: Control group (n=6). Plasma BUN, blood glucose, estrogen and progesterone hormones were estimated on 1, 4, 7, 10, 13 and 15 day post altrenogest feeding. BUN concentrations across day 1 through day 10 differed nonsignificantly (P>0.05). Across day 13, 14 and 15 Group 1 (4.6 ± 0.49 mg/dl) heifers had significantly (P<0.05) lower BUN concentration than Group 2 (5.52 ± 0.54 mg/dl) and Group 3 (7.13 ± 0.33 mg/dl). Significant differences (P<0.05) in mean plasma glucose levels were observed across day 10 and 15 in Group 1 (61.08±1.93 mg/dl), Group 3 (56.83 ± 0.41 mg/dl) and Group 2 (57.2 ± 0.36 mg/dl). Plasma Progesterone concentrations varied from 0.30 to 3.93 ng/ ml, 0.40 to 0.98 ng/ml and 0.70 to 0.94 ng/ml in Group 1, 2 and 3, respectively. Plasma progesterone in group 1 across days 4, 7, 10 and 13 differed significantly (P<0.05) from group 2 and 3.Plasma Estradiol varied from 26.32 ± 0.46 pg/ml to 40.04 ± 0.59 pg/ml, 25.42 ± 0.37 pg/ml to 37.22 ± 0.58 pg/ml and 33.41 ± 0.41 pg/ml to 34.34 ± 0.48 pg/ml in Group 1, 2 and 3, respectively. Across Day 15, Group 1 mean estradiol concentrations of 40.04 ± 0.59 pg/ml were significantly higher (P< 0.05) than mean estradiol concentrations of Group 2 (37.22 ± 0.58 pg/ml) and Group 3 (33.63 ± 0.36 pg/ml) on FTAI day. Estrogen-progesterone (EP) ratio taken as indicator of follicular growth on day 1 was (37.56 ± 1.46), (38.75 ± 0.82) and (35.84 ± 0.61) with respect to Group 1, 2 and 3 respectively. Significant differences (P< 0.05) were observed between Group 1 (134.20 ± 7.33), Group 2 (91.74 ± 3.55) and Group 3 (47.54 ± 0.81) on Day 15. EP ratio across day 4, 7, and 10 post treatment were significantly (P<0.05) lower in Group 1 and Group 2 than in Group 3 heifers. Results indicate that Altrenogest supplementation lowered BUN concentration and increased glucose concentration in prepubertal buffalo heifers. In altrenogest fed heifers the progesterone was higher in the luteal phase and the estrogen was elevated around estrous. The EP ratio was better in altrenogest fed prepubertal buffalo heifers.


Keywords: Altrenogest, Buffalo, Heifers, Hormonal levels, Metabolic profile
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How to cite this article:

Aulakh, A. S., Prahlad Singh, Gurpreet Singh, S. S. Dhindsa, M. Honparkhe and Arashdeep Kaur. 2020. Effect of Altrenogest Feeding in Prepubertal Buffalo Heifers Vis-A-Vis Plasma Estrogen-Progesterone Levels and Biochemical Profile.Int.J.Curr.Microbiol.App.Sci. 9(8): 2337-2347. doi: https://doi.org/10.20546/ijcmas.2020.908.268