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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 1935-1942
DOI: https://doi.org/10.20546/ijcmas.2020.908.221


Decolorization Potential and Comparative Laccase Activity of Pleurotus Species
Suman Kumar*, R. K. Singh and Pragya Bharti
Department of mycology & plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi – 221005, Utter Pradesh, India
*Corresponding author
Abstract:

Pleurotus spp which are already widely known for its nutrient values, four of them were collected viz., P. ostreatus, P. florida, P. sajor-caju, P. flabellatus and studies were made to detect their efficiency in terms of laccase enzyme production and dye decolorization efficiency for its industrial uses. Bromophenol blue, crystal violet, methyl red, methylene blue and brilliant blue were the five dyes mostly used on the commercial side and thus were nominated for this study on potato dextrose agar (solid medium) and potato dextrose broth (liquid medium) at three different concentrations i.e. 100ppm, 200ppm and 500ppm. The outcome at a gap of 4 days were noted down and proved Pleurotus florida as the most promising strain for decolorization of all the above mentioned dyes, in solid (PDA) as well as liquid broth media due to their lignolytic enzymes. P. sajor-caju and P. ostreatus also found to be effective for dye decolorization on solid medium as well as on liquid culture condition. P. ostreatus (96%) was found best for decolorization of bromophenol blue on solid medium and P. florida (76.33%) on liquid medium was best, for decolorization of crystal violet. Laccase activity was found to be maximum for P. florida (720.3U) during initial period and the production of laccase decreased with time, while it was reverse in case of P. ostreatus where laccase activity increased with increase in incubation periods. This study verifies the fungus to act as a biological agent to degrade synthetic dyes and for its better commercial uses.


Keywords: Pleurotus spp., Decolorization, Synthetic dyes, Laccase activity
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How to cite this article:

Suman Kumar, R. K. Singh and Pragya Bharti. 2020. Decolorization Potential and Comparative Laccase Activity of Pleurotus Species.Int.J.Curr.Microbiol.App.Sci. 9(8): 1935-1942. doi: https://doi.org/10.20546/ijcmas.2020.908.221