Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 2466-2484
DOI: https://doi.org/10.20546/ijcmas.2017.603.280


Molecular Identification and Characterization of Bacillus Antagonist to Inhibit aflatoxigenic Aspergillus flavus
A.A. Bharose, H.P. Gajera*, Darshna G. Hirpara, V.H. Kachhadia and B.A. Golakiya
Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh, 362001, Gujarat, India
*Corresponding author
Abstract:

The knowledge concerning the behavior of these Bacilli as antagonists and genetic analysis is essential for their effective use and the commercialization. The present study was focused on the analysis of the genetic diversity of rhizobacterial isolates of Bacillus using PCR based RAPD technique and selection of best biocontrol antifungal Bacillus strain with aflatoxin producing Aspergillus by antagonism on PDA medium. About 16 different strains of bacteria were isolated from healthy and infested rhizosphere of groundnut using N-agar medium. The isolates were identified based on morphological and microscopic characters such as colony color, shape, size, margin, opacity, texture, elevation, pigmentations, Gram staining and spore staining. Bacterial isolate JND-KHGn-29-A and JND-KSGn-30-L were recorded to be a best antagonist as of its ability to inhibit most toxic fungus A. flavus JAM-JKB-BHA-GG20 (58.20 %) after screening with 16 Bacillus isolates. The best antagonist bacterial isolate JND-KHGn-29-A also evidenced with nitrate reduction and sederophore as PGPR activity. The genetic diversity was studied among bacterial 16 bacterial isolates by using RAPD markers. Out of 38 primer, total 10 primers showed amplification. The highest numbers of 19 bands were produced by OPA-07 primer and lowest 1 band was produced by OPJ-07. The similarity index values generated by Jaccard’s similarity coefficient and dendrogram grouped all bacterial isolates into two main clusters at 61% similarity. The best and least bacterial antagonist were grouped into different clusters depicting genetically difference between isolates. The 16S rDNA study revealed that the best and least antagonist bacterial isolates JND-KHGn-29A and JND-KHGn-29B were identified as Bacillus subtilis.


Keywords: flavus, Aflatoxigenic, Bacillus, Antagonism, Molecular diversity, 16S rRNA gene.
Download this article as Download

How to cite this article:

Bharose, A.A., H.P. Gajera, Darshna G. Hirpara, V.H. Kachhadia and Golakiya, B.A. 2017. Molecular Identification and Characterization of Bacillus Antagonist to Inhibit aflatoxigenic Aspergillus flavus.Int.J.Curr.Microbiol.App.Sci. 6(3): 2466-2484. doi: https://doi.org/10.20546/ijcmas.2017.603.280