National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The knowledge concerning the behavior of these Bacilli as antagonists and genetic analysis is essential for their effective use and the commercialization. The present study was focused on the analysis of the genetic diversity of rhizobacterial isolates of Bacillus using PCR based RAPD technique and selection of best biocontrol antifungal Bacillus strain with aflatoxin producing Aspergillus by antagonism on PDA medium. About 16 different strains of bacteria were isolated from healthy and infested rhizosphere of groundnut using N-agar medium. The isolates were identified based on morphological and microscopic characters such as colony color, shape, size, margin, opacity, texture, elevation, pigmentations, Gram staining and spore staining. Bacterial isolate JND-KHGn-29-A and JND-KSGn-30-L were recorded to be a best antagonist as of its ability to inhibit most toxic fungus A. flavus JAM-JKB-BHA-GG20 (58.20 %) after screening with 16 Bacillus isolates. The best antagonist bacterial isolate JND-KHGn-29-A also evidenced with nitrate reduction and sederophore as PGPR activity. The genetic diversity was studied among bacterial 16 bacterial isolates by using RAPD markers. Out of 38 primer, total 10 primers showed amplification. The highest numbers of 19 bands were produced by OPA-07 primer and lowest 1 band was produced by OPJ-07. The similarity index values generated by Jaccard’s similarity coefficient and dendrogram grouped all bacterial isolates into two main clusters at 61% similarity. The best and least bacterial antagonist were grouped into different clusters depicting genetically difference between isolates. The 16S rDNA study revealed that the best and least antagonist bacterial isolates JND-KHGn-29A and JND-KHGn-29B were identified as Bacillus subtilis.
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