Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 2450-2465
DOI: https://doi.org/10.20546/ijcmas.2017.603.279


Morphological Credentials of Afla-Toxigenic and Non-Toxigenic Aspergillus Using Polyphasic Taxonomy
A.A. Bharose, H.P. Gajera*, Darshna G. Hirpara, V.H. Kachhadia and B.A. Golakiya
Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh, 362001, Gujarat, India
*Corresponding author
Abstract:

The study was performed for development of macro and micro morphological characters of Aspergillus isolated from groundnut seeds, groundnut cake and soil using cultural and microscopoic methods. Fungus colonies was inoculated in different media like Potato Dextrose Agar (PDA) and Yeast Extract Sucrose (YES) Agar and compared with standard cultures. The Aspergillus isolates grown on both medium were identified using macroscopic characteristics such as colony color, colony reverse color, colony edge, mycelia color, conidation, shape of conidia, conidial wall, mycelium growth, mycelium growth rate and microscopic characteristics including vesicle diameter, conidiophore width, length of conidia head and ascospore diameter. According to colony colour, pigmentation and length of conidial head, the 21 isolates were identified as Aspergillus species viz., A. flavus (06) (200-800µm conidial head length), A. tamarii (05) (250-700 µm), A. flavus var. columnaris (05) (500-1200 µm), A. sojae (02) (300-900 µm), A. parasiticus (01) (350-900 µm), A. niveus (01) (250-350 µm) and A. fumigatus (01) (900-950 µm). This is the first report on identification and characterization of Aspergillus strains based on length of conidial head on PDA medium. Fungal toxicity was anticipated based on aflatoxin detection using biochemical test. Out of 21, 08 isolates were found to be afla-toxigenic based on biochemical test.


Keywords: Dogs, Epidural  anaesthesia, haemodynamics, Electrocardio-graphic changes.
Download this article as Download

How to cite this article:

Bharose, A.A., H.P. Gajera, Darshna G. Hirpara, V.H. Kachhadia and Golakiya, B.A. 2017. Morphological Credentials of Afla-Toxigenic and Non-Toxigenic Aspergillus Using Polyphasic Taxonomy.Int.J.Curr.Microbiol.App.Sci. 6(3): 2450-2465. doi: https://doi.org/10.20546/ijcmas.2017.603.279