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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 2438-2449
DOI: https://doi.org/10.20546/ijcmas.2017.603.278


Development of Bioformulation and its Application against Management of Thrips and Root Rot Disease of Mulberry, Morus alba
Mohanraj1*, C.A. Mahalingam2 and R. Raghuchander3
1Department of Sericulture, The Forest College and Research Institute,
TNAU, Mettupalayam, India
2Department of Entomology, TNAU, Coimbatore, India
3Department of Plant Pathology, TNAU, Thiruvannamalai, India
*Corresponding author
Abstract:

Silkworm gut bacterial isolates were identified by using 16S rRNA gene sequence data analysis. Total viable count, spore count and spore percentage were tested on selective NA medium and recorded at different hours interval. The highest value of total viable count was recorded (420 x 108CFU/ml), spore count was higher (100 x 108CFU/ml) after 72 h of inoculation. The sporulation percentage of B. Subtilis (7.69, 10.20, 23.8 and 37.30) was found to gradually increase from 24 h to 96 h respectively. Enhancement of sporulation process using in mixture, highest total viable counts (190 ×108CFU/ml) and highest heat resistant spore count (183 ×108CFU/ ml).The spore percentage was 96.3 per cent in mixture and lowest spore percentage was observed in CaCl2 (32 ×108CFU/ml). Bacteria survived even up to 180 days of storage with different survival percentage over the period. The highest survivable percentage (88.3%) of viable cell count was recorded on 30th day. Evaluated bioformulation against pest and disease in mulberry. In thrips, highest percentage of reduction over control was observed in 100per cent (86.95%) followed by 80 per cent (80.43%), 60 per cent (75.00%) and 40 per cent (64.13%). The endogenous bacterial isolates of B. subtilis and B. tequilensis were tested individually to assess the inhibition against the radial mycelial growth of M. phaseolina and F. oxysporum. The bacterial isolates of B. subtilis recorded the least mycelial growth (45.00 mm) against M. phaseolina. The least mycelial growth of F. oxysporum was recorded in treatment with B. subtilis (63.00mm).


Keywords:

Silkworm, Gut bacteria, Thrips, Macrophomina phaseolina, Fusarium oxysporum.

 

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How to cite this article:

Mohanraj, P., C.A. Mahalingam and Raghuchander, R. 2017. Development of Bioformulation and Its Application against Management of Thrips and Root Rot Disease of Mulberry, Morus alba.Int.J.Curr.Microbiol.App.Sci. 6(3): 2438-2449. doi: https://doi.org/10.20546/ijcmas.2017.603.278