National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Nuclear DNA content and genome size variation in20 phenotypically divergent coconut accessions including 14 tall and 6 dwarfs of different geographical origin were analyzed by flowcytometry. A flow cytometric protocol amenable to the crop was standardized by comparing four buffer compositions, three sample sizes and four incubation periods for isolated nuclei using Pisium sativum cv. Citrad (2C=9.09pg) as an internal reference standard. The study revealed significant differences for 2C DNA content among the accessions ranging from 5.73pg to 6.25pg, with 8.79% difference. The mean 2C DNA content of tall accessions has6.07pg/2C and 5.89pg/2C in dwarfs, corresponding to 1C genome size of 2968.23 Mb and 2880.21Mb, respectively. Among the accessions, Guam II Tall (6.258 pg/2C) had the highest nuclear DNA content whereas Chowghat Orange Dwarf had the lowest (5.732pg/2C). The results of the present study suggest existence of intra specific variation for genome size in this species, independent of the place of origin of the distinct accessions.
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