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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 1611-1620
DOI: https://doi.org/10.20546/ijcmas.2020.908.185


Assessment of Genome Size Variation in Tall and Dwarf Coconut Accessions by Flow Cytometry
Ranjini Tantry Nagesh1*, Niral Vittal1 and K.P. Chandran2
1Division of Crop Improvement,
2Division of Social Sciences, ICAR- Central Plantation Crops Research Institute, Kasaragod, Kerala, India- 671124
*Corresponding author
Abstract:

Nuclear DNA content and genome size variation in20 phenotypically divergent coconut accessions including 14 tall and 6 dwarfs of different geographical origin were analyzed by flowcytometry. A flow cytometric protocol amenable to the crop was standardized by comparing four buffer compositions, three sample sizes and four incubation periods for isolated nuclei using Pisium sativum cv. Citrad (2C=9.09pg) as an internal reference standard. The study revealed significant differences for 2C DNA content among the accessions ranging from 5.73pg to 6.25pg, with 8.79% difference. The mean 2C DNA content of tall accessions has6.07pg/2C and 5.89pg/2C in dwarfs, corresponding to 1C genome size of 2968.23 Mb and 2880.21Mb, respectively. Among the accessions, Guam II Tall (6.258 pg/2C) had the highest nuclear DNA content whereas Chowghat Orange Dwarf had the lowest (5.732pg/2C). The results of the present study suggest existence of intra specific variation for genome size in this species, independent of the place of origin of the distinct accessions.


Keywords: Genome size, DNA content, Flow cytometry, 2C value, Intra specific variability
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How to cite this article:

Ranjini Tantry Nagesh, Niral Vittal and Chandran, K.P. 2020. Assessment of Genome Size Variation in Tall and Dwarf Coconut Accessions by Flow Cytometry.Int.J.Curr.Microbiol.App.Sci. 9(8): 1611-1620. doi: https://doi.org/10.20546/ijcmas.2020.908.185