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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 1512-1519
DOI: https://doi.org/10.20546/ijcmas.2020.908.175


Validation of Already Reported SSR Molecular Markers Linked to White Rust Resistance Gene in Indian Mustard Brassica juncea
Chandana Behera1*, D. K. Yadava2, Sujata Vasudev2, H. D. Pushpa3 and Naveen Singh2
1Department of Plant Breeding and Genetics, College of Agriculture,
Bhawanipatna, OUAT, India
2Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi-110 012, India
3ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad- 500030, India
*Corresponding author
Abstract:

White rust caused by a fungus Albugo candida, is a destructive disease of many economically important species of Brassica, to control this resistance breeding is important .Marker validation is useful for testing reported markers in determining the target phenotypes in autonomous populations and dissimilar genetic backgrounds and also involves testing the reliability of marker to predict the phenotype, which defines whether or not a marker could be used in routine screening for MAS. The current study was carried out with the objective to validating the already reported intron polymorphic marker (At 5g 41560 and At 5g 41940) from Arabidopsis at 1.2 cM from AcB1-A4.1. 102 RIL population derived from Varuna x Bio YSR of Brassica juncea were validated along with their parents. Phenotypic and genotypic data of RIL population confirmed At5g41560 marker was polymorphic to both the parents as well as differentiate the resistance and susceptible individual of the population and found to be validated. But At5g41940 was found to be not validated.  The present study indicated that At5g41560 marker could be used for screening the resistance and susceptible genotypes in oilseed breeding programs. For the introgression of white rust resistance gene, at 5g 41560 marker could be used in MAS as it was not specific to genotype.


Keywords: Brassica juncea, Validation, SSR marker, White rust, Albugo candida
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How to cite this article:

Chandana Behera, D. K. Yadava, Sujata Vasudev, H. D. Pushpa and Naveen Singh. 2020. Validation of Already Reported SSR Molecular Markers Linked to White Rust Resistance Gene in Indian Mustard Brassica juncea.Int.J.Curr.Microbiol.App.Sci. 9(8): 1512-1519. doi: https://doi.org/10.20546/ijcmas.2020.908.175