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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 1355-1365
DOI: https://doi.org/10.20546/ijcmas.2020.908.154


Sero-prevalence of Dirofilaria repens Infection in Dogs by Indirect-ELISA using Microfiarial and Adult Worm Antigen
S. Malatesh*, C. Ansar Kamran, K. J. Ananda and G. B. Manjunath Reddy
Department of Veterinary Medicine, Veterinary College, Shivamogga- 577 204, Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar, Karnataka, India
*Corresponding author
Abstract:

Dirofilariosis is vector borne parasitic diseases of dogs caused by filarial nematodes of the genus Dirofilaria, which includes more than 40 different species. Among them D. immitis causes heartworm disease, whereas D. repens causes subcutaneous dirofilariosis in dogs. A study was conducted to determine seroprevalence of D. repens in dog from Shivamogga and Mangalore regions of Karnataka using Indirect-Enzyme Linked Immuno Sorbent Assay (Indirect-ELISA). A total of 315 dog blood samples were collected for detection of microfilaria by modified Knott's method and serum samples of the same dogs were screened for antibodies of D. repens by Indirect-ELISA using microfilarial and adult worm antigen. Among 315 serum samples screened, 220 and 183 were showed antibodies to microfilarial and adult worm antigen respectively. The seroprevalence of D. repens in dogs recorded in the present study using microfilarial and adult worm antigen was 69.84 and 58.09 per cent respectively. The sensitivity and specificity of Indirect ELISA with microfilarial antigen was found 100 and 64.09 per cent whereas, with adult worm antigen 100 and 72 per cent respectively. This form the first report that, the microfilarial and adult worm antigen of D. repens was used for the detection antibodies of D. repens in dog by indirect- ELISA from Karnataka. The present study showed that, the microfilarial antigen was found more sensitive compared to adult worm antigen in detection antibodies of D. repens in dog. The result indicates that, the microfilarial antigen can be effectively used for seroprevalence study at field level for large population.


Keywords: Sero-prevalence, D. repens, Dogs, Indirect-ELISA, Shivamogga, Mangalore
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How to cite this article:

Malatesh, D. S., C. Ansar Kamran, K. J. Ananda and Manjunath Reddy, G. B. 2020. Sero-prevalence of D. repens Infection in Dogs by Indirect-ELISA using Microfiarial and Adult Worm AntigenInt.J.Curr.Microbiol.App.Sci. 9(8): 1355-1365. doi: https://doi.org/10.20546/ijcmas.2020.908.154