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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 1333-1342
DOI: https://doi.org/10.20546/ijcmas.2020.908.151


RAPD and SSR Based Genetic Diversity in Pistillate Parents of Castor (Ricinus communis L.) Germplasms
R. Aher*, M. S. Kamble, A. G. Bhoite and M. S. Mote
Agricultural Botany Division, RCSM College of Agriculture, Kolhapur 416004
(University: Mahatma Phule Krishi Vidyapeeth, Rahuri, MAHARASHTRA), India
*Corresponding author
Abstract:

This study aimed to evaluate the genetic diversity and to characterize nine pistillate parents of castor (Ricinus communis L.) germplasms by RAPD and SSR markers. The availability of diverse germplasm of any crop is an important genetic resource to mine the genes that may assist in attaining yield as well as quality. The genomic DNA result of germplasms was amplified with forty nine oligonucleotide primers and twenty eight microsatellite primer pairs for RAPD and SSR assay, respectively. In RAPD analysis percent polymorphism ranged from 12.50% to 100% with an average percent polymorphism of 67.56. The PIC values varied from 0.55 to 0.93 with an average PIC value 0.844. RAPD-Dendogram divided pistillate parents into two main clusters A and B, with Jaccard’s similarity coefficient ranging from 0.67 to 0.85. In SSR analysis, 28 primers generated 67 alleles with average of 2.39 alleles per primer. Simple sequence repeats analysis revealed 100% polymorphism with different fragments size; ranged from 138bp to 311bp. The number of alleles in individual primer was ranged from two to four with an average of 0.43. The PIC values obtained in this study were substantially higher, which could be attributed due to high genotypic diversity of 9 castor genotypes. Clustering pattern of dendogram generated by pooled SSR data showed two major clusters A and B having similarity coefficient of 0.12 to 0.75. Clustering of these pistillate lines was in accordance with their pedigree. Comparative account of RAPD and SSR markers showed that RAPD marker system was found to be superior to SSR markers. Even though SSR markers are coined to be superior to RAPD markers, identification of low number of alleles with SSR markers might be a possible reason that explains these contrasting results.


Keywords: RAPD, SSR, Castor, Ricinus communis L., Genetic diversity
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How to cite this article:

Aher, A. R., M. S. Kamble, A. G. Bhoite and Mote, M. S. 2020. RAPD and SSR Based Genetic Diversity in Pistillate Parents of Castor (Ricinus communis L.) Germplasms.Int.J.Curr.Microbiol.App.Sci. 9(8): 1333-1342. doi: https://doi.org/10.20546/ijcmas.2020.908.151