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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 1060-1067
DOI: https://doi.org/10.20546/ijcmas.2020.908.116


Assessment of Genetic Diversity among Poplar (Populus deltoides Marsh.) Clones from India using RAPD Markers
Rajeev Kumar1, Bimlendra Kumari1*, Shikha Yashveer2 and Prashant Kaushik3*
1Department of Forestry, CCS Haryana Agricultural University,
Hisar-125 004, Haryana, India
2Department of Molecular Biology, Biotechnology and Bioinformatics, CCS Haryana Agricultural University, Hisar-125 004, Haryana, India
3Instituto de Conservación y Mejora de la Agrodiversidad Valenciana, Universitat Politècnica de València, 46022 Valencia, Spain
*Corresponding author
Abstract:

Here we determined the genetic diversity among 19 Populus deltoides clones collected from forest nurseries of four districts of Haryana State. All of these were raised in the farm area of the Department of Forestry, and the leaf samples from the young plants of different clones of poplar were collected for molecular analysis. The extraction method (CTAB), DNA purification and, PCR reaction conditions were standardized to obtain genetic diversity.Out of the 30 RAPD markers used in this study, only 11 showed polymorphic pattern and showed a total of 94 alleles. Out of these, 59 were polymorphic, and 35 were monomorphic. An average number of alleles amplified was 8.54. The genetic similarity value calculated varied from 0.20 to 0.73 for 19 Poplar clones.The maximum similarity value (0.73) was observed between clone FRIAM 100 and W32, indicating FRIAM 100 and W32 to be most closely related genotypes.Despite low number of alleles that detected polymorphism, RAP Danalysis indicated that there is high genetic diversity among Populus deltoides clones analyzed in this study. Since poplar is a crucial commercial agroforestry tree of Haryana state, this type of genetic characterization of the planting material is a pre-requisite to ensure a broader genetic base of the species.


Keywords: Genetic diversity, Populus deltoides clones, Similarity index, RAPD markers
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How to cite this article:

Rajeev Kumar, Bimlendra Kumari, Shikha Yashveer and Prashant Kaushik. 2020. Assessment of Genetic Diversity among Poplar (Populus deltoides Marsh.) Clones from India using RAPD Markers.Int.J.Curr.Microbiol.App.Sci. 9(8): 1060-1067. doi: https://doi.org/10.20546/ijcmas.2020.908.116