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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 1035-1042
DOI: https://doi.org/10.20546/ijcmas.2020.908.113


Occurrence, Molecular Characterization and PhysiologicalStudy of Broccoli Infected with Turnip mosaic virus (TuMV)  in Arunachal Pradesh, India
Raghuveer Singh1*, Amrita Banerjee2, Susheel Kumar Sharma3 and Valenta Kangjam4
1ICAR Research Complex for NEH Region, Arunachal Pradesh Centre, Basar-791101, India
2ICAR-NRRI, Central Rainfed Upland Rice Research Station,
Hazaribag (Jharkhand)-825301, India
3ICAR Research Complex for NEH Region, Manipur Centre,
Lamphelpat (Imphal)-795004, India
4Department of Plant Pathology, SASRD, Nagaland University, Medziphema-797106, India
*Corresponding author
Abstract:

Broccoli (Brassica oleracea var. italica) is an important part of diet in India. Turnip mosaic virus (TuMV), member of the genus Potyvirus, is the most important virus of commercially grown broccoli in many Asian countries. Symptoms based survey of different backyard vegetable gardens during rabi season of 2015-16, revealed that the mean disease incidence of TuMV was 36.21 per cent. The occurrence of TuMV in Broccoli was confirmed by symptomatology, transmission electron microscopy (flexuous filamentous particles of 800 × 12 nm), DAS-ELISA, RT-PCR and partial characterization of cytoplasmic inclusion (CI) protein and coat protein (CP) domains. Phylogenetic analysis of the partial CP sequences of the new TuMV isolate (AR-Brc; KP876504) revealed their closest relationship with the members of the World-B genogroup of TuMV. Significant physiological changes were observed in diseased leaves in terms of chlorophyll, total sugar, reducing sugar, total phenol and total proteins. There was significant decrease in chlorophyll contents; chlorophyll-a (48.75%), chlorophyll-b (42.86%) and total chlorophyll (47.77%). Total protein content significantly increased in case of severe symptoms (22.55%) followed by mild symptoms (19.61%). There was a significant increase in the total sugar content in mild symptom (60%) followed by severe symptoms (20%). Significant increase in the reducing sugar content was also observed in mild symptoms (5.88%) whereas it decreased in severe symptoms (11.76%). Similarly, total phenol content also significantly increased in mild type (9.09%) whereas it decreased in severe symptoms (13.63%). 


Keywords: TuMV, Transmission electron microscopy, DAS-ELISA, RT-PCR, Phylogeny
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How to cite this article:

Raghuveer Singh, Amrita Banerjee, Susheel Kumar Sharma and Valenta Kangjam. 2020. Occurrence, Molecular Characterization and Physiological Study of Broccoli Infected with Turnip mosaic virus (TuMV) in Arunachal Pradesh, India.Int.J.Curr.Microbiol.App.Sci. 9(8): 1035-1042. doi: https://doi.org/10.20546/ijcmas.2020.908.113