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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 912-921
DOI: https://doi.org/10.20546/ijcmas.2020.908.099


Molecular and Phenotypic Assay of Improved Lines Harboring Bacterial Blight Resistance
Aravindhan1, 2, C. Vanniarajan3, P. Meenakshi Sundaram1 and J. Ramalingam1,2*
1Department of Plant Biotechnology, Center for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, India
2Center of Innovation, Dept. of Biotechnology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai, India
3Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai, India
*Corresponding author
Abstract:

Bacterial blight (BB) is one of the serious and wide spread diseases of many rice cultivating areas in India as well as world. Development of cultivars pyramided with more than two BB resistance genes increases durable resistance to prevailing and emerging biotypes and it offers environment safe approach to manage the disease. The present study was carried out to screen the improved lines with the foreground markers of Xa21, xa13, xa5, Xa33 and Xa38 and the confirmed plants were evaluated for phenotypic resistance against the local isolate of BB under the controlled conditions. Further, the lines harboring BB resistance genes were evaluated for key-morphological traits to identify the superior genotypes. The bioassay revealed, most of the lines harboring Xa21, xa13, xa5andXa33/Xa38 in homozygous conditions were displayed high degree of resistance to bacterial blight with a mean lesion length (LL) ranged from 2.13 ± 0.41to 2.92 ± 0.65. The bioassay results also suggest, the lines harboring Xa21+ xa13 + xa5 + Xa33 resistance genes were observed to have less lesion length (2.13 ± 0.45) than Xa21+ xa13 +xa5 + Xa38 resistance genes (2.81 ± 0.40). Cluster analysis of 24 improved lines based on co-efficient of genetic distance, identified the superior genotypes, which posses morphological characters as recurrent parents and also enriched with BB resistance genes.


Keywords: Bacterial blight, Molecular markers, Phenotypic evaluation, Agro-morphological characters, Cluster analysis
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How to cite this article:

Aravindhan, P., C. Vanniarajan, P. Meenakshi Sundaram and Ramalingam, J. 2020. Molecular and Phenotypic Assay of Improved Lines Harboring Bacterial Blight Resistance.Int.J.Curr.Microbiol.App.Sci. 9(8): 912-921. doi: https://doi.org/10.20546/ijcmas.2020.908.099