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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 775-782
DOI: https://doi.org/10.20546/ijcmas.2020.908.083


Identification of Staphylococcus pseudintermedius and MRSP in Dogs with Pyoderma by PCR
Soumya¹, M. A. Kshama²*, Srikrishna Isloor³ and Veeresh B. Hanchinal3
1Veterinary Officer, Shimoga, Karnataka, India
2Department of Veterinary VCC, Veterinary College, Bangalore, India
3Department of Veterinary Microbiology, Veterinary College, Bangalore, India
*Corresponding author
Abstract:

Pyoderma is one of the most common dermatological disorders encountered in small animal practice. Almost ninety per cent of the cases of pyoderma in dogs is caused by bacteria belonging to the genus Staphylococcus and now Staphylococcus pseudintermedius is recognized as the main etiological agent responsible for pyoderma Further, Methicillin Resistant S pseudintermedius is being reported worldwide and represents a serious threat to the health of dogs. However not much work on S pseudintermedius has been done in India. Hence the study, with the primary objective being isolation, identification and genotypic characterization of staphylococcal organisms responsible for pyoderma in dogs along with detection of genotypic resistance to methicillin in these organisms. Dogs presented to the Veterinary College Hospital, Bangalore with clinical signs suggestive of pyoderma were chosen as subjects for the study. Materials from lesions from these dogs were  collected, processed and cultured in Mannitol Salt Agar. DNA extracted from these isolates were subjected to multiplex PCR using species specific primers and were also tested for methicillin resistance genotypically by PCR  targeting the mecA gene. All the twenty isolates were staphylococci and eleven of the isolates were found to be S pseudintermedius and nine were S aureus. Sixteen of the twenty isolates were resistant to methicillin genotypically on PCR. The study conclusively proved the occurrence of S pseudintermedius in dogs with pyoderma which probably has not been reported from India. Further, it also proved the occurrence of methicillin resistant S pseudintermedius and S aureus in dogs with pyoderma which could be important from the perspective of therapeutic management of pyoderma


Keywords: Pyoderma, Staphylococci,pseudintermedius, Methicillin Resistant S pseudintermedius (MRSP),mecA gene
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How to cite this article:

Soumya, B., M. A. Kshama, Srikrishna Isloor and Veeresh B. Hanchinal. 2020. Identification of Staphylococcus pseudintermedius and MRSP in Dogs with Pyoderma by PCR.Int.J.Curr.Microbiol.App.Sci. 9(8): 775-782. doi: https://doi.org/10.20546/ijcmas.2020.908.083