National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The bacterial strain a thermophilic carboxymethyl cellulase-producing was screened from biocompost. The strain was identified as Bacillus sonorensis CY-3 according to morphological, biochemical and molecular analysis, then it was optimized for the production of carboxymethyl cellulase (CMCase). Enzyme was optimally produced in a Luria Broth (LB) medium containing carboxymethyl cellulose (CMC) at pH 9.0 and 55ºC. Two bands were found with molecular mass 80 kDa and 72 kDa by use SDS-PAGE and the Vmax and Km were measured 380.19 U/ml and 5.82 mg/ml, respectively. The partially purified enzyme has showed optimal activity at pH 9.0 and 100°C while it was stabled from pH 6.0 to 13.0 with more than 65% activity. It was found to have the properties of enzyme highly thermostability, pH stability, and stability in the presence of some additives that made potentially useful in textile, laundry, and other industrial applications.
Citations |
 |
 |
 |
 |
