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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 550-556
DOI: https://doi.org/10.20546/ijcmas.2020.908.062


Identification of SNP in Lipoprotein Lipase Gene and its Validation and Association with Milk Production in Goats
Mathivathani*, G. Radhika and T. V. Aravindakshan
Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Mannuthy, Thrissur – 680 651, India
*Corresponding author
Abstract:

In Kerala, goat farming forms an important source of income and nutritional security to the rural poor and unemployed sections of society. Milk production, is one of the important economic trait in goats. In Kerala, two native goat breeds, Malabari and Attappady Black vary significantly in milk production. Double digest Restriction Associated DNA Sequencing (ddRADseq) is a modern approach for identification of Single Nucleotide Polymorphisms (SNP) across populations. This ddRADseq technique was performed in three pooled samples (ten each) of high and low milk producing Malabari and Attappady Black goats. The SNPs discovered were analysed using bioinformatics tools. A total of 42 candidate genes affecting milk quantity and quality were selected from literature and the entire data was screened for variants in these candidate genes. Out of these candidate genes, LPL gene (LPL)was selected for validation in the present study as researches showed that LPL polymorphism was found to be significantly associated with milk yield (kg) in goats. In the current study, Intronic variant c.243 + 1082C>T LPL, was validated using Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) and further sequencing. It had significant (p≤0.05) association with daily average milk yield and CT (494.72 ± 11.60 mL) genotype showed higher milk yield. Current study emphasized the relevance of ddRADseq in identifying novel SNP in candidate genes influencing economic traits in goats.


Keywords: Milk production, Malabari, Attappady Black, ddRADseq, SNP, PCR-SSCP
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How to cite this article:

Mathivathani, K., G. Radhika and Aravindakshan, T. V. 2020. Identification of SNP in Lipoprotein Lipase Gene and its Validation and Association with Milk Production in Goats.Int.J.Curr.Microbiol.App.Sci. 9(8): 550-556. doi: https://doi.org/10.20546/ijcmas.2020.908.062