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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 467-473
DOI: https://doi.org/10.20546/ijcmas.2020.908.055


Muscle Total Lipid, Total Protein, Total Antioxidants, Co Q and Mitochondrial Concentration Analysis in Coq10 Supplemented Broiler Chicken
Ramavathi*, P. Selvaraj, P. Visha and V. Thavasiappan
Department of Veterinary Physiology, VCRI, Namakkal, India
*Corresponding author
Abstract:

Two hundred and forty (240) numbers of day old broiler straight run chicks were wing banded, weighed and randomly allotted to 5 groups 6 replicates of eight chicks each based on the body weight. The treatments were, Basal diet without CoQ10 supplementation, Low energy diet without CoQ10 supplementation, Low energy diet with 20mg of CoQ10 /kg diet, Low energy diet with 40mg of CoQ10 /kg diet, Low energy diet with 60mg of CoQ10 /kg diet. Muscle total lipid was estimated gravimetrically by using Folch method of lipid extraction. The antioxidant ability of muscle was determined by the method described by Benzie and Strain. One gram of muscle tissue was taken and CoQ10 was extracted by using solvents methanol:hexane. Protein content of meat was estimated by kjeldhal method according to procedure described in AOAC. Hepatic mitochondria were obtained by differential centrifugation as outlined by Cawthon et al., (1999). Mitochondrial protein concentration was estimated as per the method of Lowry et al., 1971. There was a significant difference in protein content of the breast muscle was observed between treatment groups and control. The protein accretion on the muscle ranged from 7 to 9%. The result of the present study agrees with the average value of 22% in breast muscle of broiler chicken.The effect of CoQ10 on muscle lipid did not exhibit any variation. It is understood from the result that CoQ10 on lipid accretion in muscle is negligible. The results of the study clearly proved that antioxidants level was influenced by the level of CoQ10 in the diet. Further the present study also shown that antioxidants status was lower in birds fed less energy in the diet. Our findings agrees with many earlier observations (Littarru et al., 2007), Mates et al., (1999), Kapoor and Kapoor (2013) and Fathi (2015) on antioxidants status due to supplementation of CoQ10. There was no significant difference existed in the mean breast muscle CoQ10 for T3 and T4 group of birds. The mean breast muscle CoQ10 was significantly (P<0.05) lower in low energy diet without CoQ10 supplemented group (T2). The mean muscle mitochondrial protein concentration was significantly (P<0.05) higher in the CoQ10 supplemented group of birds (T3, T4 and T5) in comparison to T2 and groups. There was no significant difference in the mean mitochondrial protein concentration of T3, T4 and T5 groups of chicken.


Keywords: Total Lipid, Protein, Antioxidants, Co Q and Mitochondrial concentration
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How to cite this article:

Ramavathi, J., P. Selvaraj, P. Visha and Thavasiappan, V. 2020. Muscle Total Lipid, Total Protein, Total Antioxidants, Co Q and Mitochondrial Concentration Analysis in Coq10 Supplemented Broiler Chicken.Int.J.Curr.Microbiol.App.Sci. 9(8): 467-473. doi: https://doi.org/10.20546/ijcmas.2020.908.055