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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(3): 15-25
DOI: http://dx.doi.org/10.20546/ijcmas.2016.503.003


Evaluation of Antiulcer Activity of Commiphora africana (A. Rich) Engl. (Burseraceae) Stem-bark Extracts Using Ethanol Induced Ulcer Model in Albino Rats
A. Nuhu1*, U. H. Danmalam1, N. Ilyas1, A. M. Zakariya1, S. Shehu2 and U. M. Jajere1
1Department of Pharmacognosy and Drug Development, Ahmadu Bello University Zaria-Nigeria
2Department of Pharmacognosy and Ethnopharmacy, Usman Danfodiyo University Sokoto Nigeria
*Corresponding author
Abstract:

This study was designed to evaluate the antiulcer activity of Commiphora africana stem-bark extracts using ethanol induction model in laboratory rats and to identify phytochemical constituents of the extracts responsible for the observed activity. C. africana stem-bark was extracted with n-hexane, ethyl acetate and methanol gradient wise in a soxhlet apparatus at 50ºC. The extracts were subjected to qualitative phytochemical analysis, toxicity experiment and anti- ulcer evaluation using ethanol – induced gastric ulcer in laboratory rats. A standard anti-ulcer agent, omeprazole was used as reference standard. The data were analyzed by one-way Analysis of Variance with significant level at (p<0.05). The percentage yield from the gradient extraction of C. africana stem-bark showed methanol to be the highest. Thin Layer Chromatographic analysis visualized with specific reagents confirmed the presence of steroids/triterpenes, phenolic compounds and flavonoids in the stem-bark of C. africana. LD50 was above 5000 mg/kg and did not cause mortality in all the tested rats. Ethanol triggered severe gastric ulcers with mean ulcer index (12.8 ± 0.97 mm) and pretreatment with C. africana stem-bark  n-hexane, ethyl acetate and methanol extracts at (250, 500 and 1000 mg/kg) and Omeprazole (20 mg/kg) produced a significant (p<0.05) dose dependent anti-ulcer activity with increase in percentage ulcer inhibition of (9.38%, 43.75% and 57.81%) for CAHE, (59.38%, 68.75% and 85.94%) for CAEE and (65.63%, 73.44% and 90.63%) for CAME respectively. Omeprazole had 68.75% ulcer inhibition. This study demonstrated that C. africana has anti-ulcer potential and it justified the traditional uses of the stem-bark in ulcer treatment.


Keywords: Commiphora africana, TLC, Acute toxicity, Antiulcer activity,Ethanol.
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How to cite this article:

Nuhul, A, Danmalam, U. H., Ilyas, N., Zakariya1, A. M., Shehu, S., Jajere, U. M. 2016. Evaluation of Antiulcer Activity of Commiphora africana (A. Rich) Engl. (Burseraceae) Stem-bark Extracts Using Ethanol Induced Ulcer Model in Albino Rats.Int.J.Curr.Microbiol.App.Sci. 5(3): 15-25. doi: http://dx.doi.org/10.20546/ijcmas.2016.503.003