Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 341-351
DOI: https://doi.org/10.20546/ijcmas.2020.908.040


Circulating MicroRNA-21-3p: A Potential Biomarker for Peste-des petits ruminants Virus in Naturally Infected Goats
Preeti P. Bramhapurkar1, Prabhakar A.Tembhurne1*, S. Chandra Sekar3,Muthucheven3, Sharvan Sehrawat4, Prashant Tarale1,Vijay.C.Ingle1 and Rajeev Kaul2
1Department of Veterinary Microbiology and Animal Biotechnology, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur-440006, Maharashtra, India
2University of Delhi, South Campus, New Delhi, India
3Indian Veterinary Research Institute (IVRI), Mukteshwar, Nainital, Uttarakhand, India
4Indian Institute of Science Education and Research, Mohali, Punjab, India
*Corresponding author
Abstract:

Micro RNAs have been recognized to play vital role in viral replication and pathogenesis. Circulatory miRNAs found in body fluid in stable, cell free form may be correlated with different stages of Peste des Petits Ruminanats (PPR) infection.The present study was focused to profile expression of circulatory miR-21-3p in serum of PPRV infected and apparently healthy goats in natural condition. The identification of suitable endogenous miRNA in serum samples and miRNA-21-3p profiling was performed using quantitative real time PCR (qRT-PCR) in 20 representative samples of PPRV infected goats from four different outbreaks (Bondri, Nagpur, Umred and Yawatmal district) in Maharashtra state, India during year January 2017-December 2017. The miR-16 was identified as endogenous control while expression of miR-21-3p was significantly elevated in all 20 serum samples of PPRV infected goats than control group with fold change ranging from 1.99 to 31.77 (p value ≥ 0.05) in PPR infected samples. Relative fold change values varied in infected samples corresponding to symptoms shown by infected animals. We predicted miR-21-3p may be used as indicator for stage of PPRV infection and as promising biomarker for PPRV disease progression.


Keywords: PPRV, serum miRNA ,miR-21-3p, viral pathogenesis, bBomarker,Disease progression
Download this article as Download

How to cite this article:

Preeti P. Bramhapurkar, Prabhakar A.Tembhurne, S. Chandra Sekar, D. Muthucheven, Sharvan Sehrawat, Prashant Tarale, Vijay.C.Ingle and Rajeev Kaul. 2020. Circulating MicroRNA-21-3p: A Potential Biomarker for Peste-des petits ruminants Virus in Naturally Infected Goats.Int.J.Curr.Microbiol.App.Sci. 9(8): 341-351. doi: https://doi.org/10.20546/ijcmas.2020.908.040