National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Micro RNAs have been recognized to play vital role in viral replication and pathogenesis. Circulatory miRNAs found in body fluid in stable, cell free form may be correlated with different stages of Peste des Petits Ruminanats (PPR) infection.The present study was focused to profile expression of circulatory miR-21-3p in serum of PPRV infected and apparently healthy goats in natural condition. The identification of suitable endogenous miRNA in serum samples and miRNA-21-3p profiling was performed using quantitative real time PCR (qRT-PCR) in 20 representative samples of PPRV infected goats from four different outbreaks (Bondri, Nagpur, Umred and Yawatmal district) in Maharashtra state, India during year January 2017-December 2017. The miR-16 was identified as endogenous control while expression of miR-21-3p was significantly elevated in all 20 serum samples of PPRV infected goats than control group with fold change ranging from 1.99 to 31.77 (p value ≥ 0.05) in PPR infected samples. Relative fold change values varied in infected samples corresponding to symptoms shown by infected animals. We predicted miR-21-3p may be used as indicator for stage of PPRV infection and as promising biomarker for PPRV disease progression.
Citations |
 |
 |
 |
 |
