National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
High-quality RNA extraction are very important for further downstream molecular applications such as transcript amplifications by reverse transcriptase-polymerase chain reaction (RT-PCR) and elaboration of cDNA for expressed gene study. Aspergillus flavus is the paramount aflatoxin producing fungus in cultivated oil seeds. The methodology with some modification is described here that allows the intact RNA isolation from mycelium. RNA quality was evaluated by electrophoresis in agarose gel, quantitative Real-Time PCR of cDNAs derived from isolated mRNA and subsequent PCR amplification using primers designed against β-tubulin and aflO/ omtB/ dmtA/ O methyltransferase B from A. flavus.
Citations |
 |
 |
 |
 |
