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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 325-332
DOI: https://doi.org/10.20546/ijcmas.2020.908.038


Optimization of CTAB-based RNA Extraction Method for Aspergillus flavus
Department of Biotechnology, Junagadh Agricultural University, Junagadh, Gujarat, India
Department of Biotechnology, Junagadh Agricultural University, Junagadh, Gujarat, India
*Corresponding author
Abstract:

High-quality RNA extraction are very important for further downstream molecular applications such as transcript amplifications by reverse transcriptase-polymerase chain reaction (RT-PCR) and elaboration of cDNA for expressed gene study. Aspergillus flavus is the paramount aflatoxin producing fungus in cultivated oil seeds. The methodology with some modification is described here that allows the intact RNA isolation from mycelium. RNA quality was evaluated by electrophoresis in agarose gel, quantitative Real-Time PCR of cDNAs derived from isolated mRNA and subsequent PCR amplification using primers designed against β-tubulin and aflO/ omtB/ dmtA/ O methyltransferase B from A. flavus.


Keywords: Extraction; Fungi; Real time; PCR; RNA
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How to cite this article:

Virali Antala, Hemangini Chaudhari, Nidhi Radadiya, Hiral Desai, B. A. Golakiya and Gajera, H. P. 2020. Optimization of CTAB-based RNA Extraction Method for Aspergillus flavus.Int.J.Curr.Microbiol.App.Sci. 9(8): 325-332. doi: https://doi.org/10.20546/ijcmas.2020.908.038