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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 223-230
DOI: https://doi.org/10.20546/ijcmas.2020.908.025


Isolation of Listeria monocytogenes and Listeria ivanovii and its Nitric Oxide Expression Level in Serum, Brain and Reproductive Organs of Sheep
Nitasha Sambyal1, Varun Bassessar2, Rahul Singh2*, Nafia Rufai3 and Bharat Mengi4
1Department of Veterinary Public Health and Epidemiology, 2Department of Veterinary Pathology, Khalsa College of Veterinary and Animal Sciences, Amritsar (143002), India
3Sher-e-Kashmir University of Agricultural Sciences and Technology,
Jammu (180016), India
4Obihiro University of Agriculture and Veterinary Medicine (OUAVM), Hokkaido, Japan
*Corresponding author
Abstract:

Two species of Listeria are pathogenic; L. monocytogenes infects humans and animals, and L. ivanovii infects ruminants only have been considered. While infection with Lmonocytogenes and Livanovii are known as important causes of abortion and reproductive failure in ruminants and the role of nitric oxide (NO) expression in different organs of affected animals in India is limited. Therefore, the current study, (n= 78) serum and organs (comprising of 26 ovaries, 26 uterus and 26 brain) samples from the local abattoir in and around Jammu region were taken and evaluate for the presences of Listeria spp. by employing two fold enrichment procedure for isolation and confirmed by biochemical tests. Then further, pathogenicity of isolated were confirmed by Phosphatidylinositol-specific phospholipase C (PI-PLC) assay. Out of them L. monocytogenes was detected in 5 samples i.e. 3 in uterus, 1 in ovary and 1 in brain, and L. ivanovii was isolated only in 3 uterus sample with over-all prevalence of 6.41% and 3.85% respectively. The prevalence of other Listeria spp. in the sample was 3.85%. Further, we were found high concentration of NO in Listeria spp. infected samples in comparison to the Listeria spp. was not isolated. This study demonstrated that Listeria spp. (mainly L. monocytogenes and L. ivanovii) infected sheep showed very high NO concentration level compared to without infected animals. The functional role of nitric oxide (either iNOS or nNOS) needs to be further studied in natural and experimental models with Listeria spp. infection.


Keywords: Listeria ivanovii, L. monocytogenes, Sheep, Prevalence
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How to cite this article:

Nitasha Sambyal, Varun Bassessar, Rahul Singh, Nafia Rufai and Bharat Mengi. 2020. Isolation of Listeria monocytogenes and Listeria ivanovii and its Nitric Oxide Expression Level in Serum, Brain and Reproductive Organs of Sheep.Int.J.Curr.Microbiol.App.Sci. 9(8): 223-230. doi: https://doi.org/10.20546/ijcmas.2020.908.025