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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 4273-4282
DOI: https://doi.org/10.20546/ijcmas.2020.906.501


Study on Skin Sensitization Potential of an Anti-tumor necrosis factor -alpha receptor fusion protein in Guinea pigs
G. Selvam1*, S. Bhuvana2, P.C. Prabu3 and K. Sadasivan Pillai1
1University of Madras, Chepauk, Chennai – 600 005, India 2Department of Academics and Human Resource Development, Indian Institute of Food Processing Technology, Thanjavur – 613 005, India  3Department of Veterinary Pathology, Madras Veterinary College, Tamilnadu Veterinary and Animal Sciences University, Chennai – 600 007, India
*Corresponding author
Abstract:

Etanercept is an anti-TNFα biopharmaceutical used in the treatment of several disorders of inflammation as well as autoimmune conditions. This present study was aimed to identify the skin sensitization potential of the etanercept, in guinea pigs by following Guinea Pig Maximization Test (GPMT) of Magnusson and Kligman method. The animals were divided into two groups; negative control comprising six males and 6 females and treatment groups having ten males and ten females. Through a range finding study, the dose for main study was selected as 0.5% (low concentration) and 1.0% (high concentration) of etanercept for topical induction and challenge doses. The application site was examined at 48 and 72 hours post-dosing for the presence of allergic reactions. Apart from this, animals were observed for body weight and exhibition of clinical signs of toxicity daily throughout the experimental period. At both time points of observation, no skin reaction was observed in the experimental group after the challenge. The body weights of the treated animals were found to be comparable with the control animals. Based on the results of the study, the etanercept was found to be safe and well tolerated and was concluded to be a non- sensitizer to the skin of the guinea pigs.


Keywords: Anti-TNFα biopharmaceuticals, skin sensitization, preclinical safety, guinea pigs
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How to cite this article:

Selvam, G., S. Bhuvana, P.C. Prabu and Sadasivan Pillai, K. 2020. Study on Skin Sensitization Potential of an Anti - tumor necrosis factor -alpha receptor fusion protein in Guinea pigs.Int.J.Curr.Microbiol.App.Sci. 9(6): 4273-4282. doi: https://doi.org/10.20546/ijcmas.2020.906.501