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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 3776-3788
DOI: https://doi.org/10.20546/ijcmas.2020.907.442


Characterization of Plant Growth Promoting Rhizobial Isolates for Pigeon Pea (Cajanus cajan [L.] Mill sp)
A. Harshitha1*, B.Geeta. Goudar2, P. U. Krishnaraj2 and R. V. Koti3
1Department of Agricultural Microbiology, College of Agriculture, Vijayapur,
2Department of Agricultural Microbiology, College of Agriculture, Dharwad,
3Department of Crop physiology, College of Agriculture, Dharwad, University of Agricultural Sciences, Dharwad, Karnataka, India
*Corresponding author
Abstract:

Study was conducted to isolate and characterize Rhizobia from root nodules of pigeon pea from Vijayapur, Kalaburgi and Bagalkot districts of northern regions of Karnataka. Ten Rhizobial isolates and reference strain PPM35B were subjected for nodulation test. All the isolates showed positive result for nodulation. The number of nodules was ranging from 12 to 14.5 per plant. All these isolates were also subjected for functional characterization. The amount of IAA, GA production and ACC deaminase activity by different rhizobial isolates ranged from 16.60 to 22.85 g IAA/ ml of broth, 11.00 to 14.43 g/25ml of broth and 44.5 to 73.5 nmoles of α-ketobutyrate/mg/h of broth respectively. The diameter of P, Zn and Si solubilization by the rhizobial isolates was ranged from 4.5 to 10.3 mm, 9.5 to 20.5 mm and 5.5 to 6.5 mm, respectively. These isolates were further subjected for morphological and biochemical characterization. All the isolates were found to be rod shaped and gram negative. All the isolates were positive for citrate utilization, catalase, urease, oxidase, acid and gas production tests whereas negative for Voges proskauer’s and indole production test.


Keywords: Rhizobium, PGPR, IAA, P-solubilization
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How to cite this article:

Harshitha, B. A., Geeta. Goudar, P. U. Krishnaraj and Koti, R. V. 2020. Characterization of Plant Growth Promoting Rhizobial Isolates for Pigeon Pea (Cajanus cajan [L.] Mill sp).Int.J.Curr.Microbiol.App.Sci. 9(7): 3776-3788. doi: https://doi.org/10.20546/ijcmas.2020.907.442