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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 3748-3755
DOI: https://doi.org/10.20546/ijcmas.2020.907.439


A Probe can Capture Circulating Tumor Cells (CTC) – An Antitumor Antibody based Capture Technique
Karthikeyan Ramaiyan1, Pandiyan Velayutham1,T. M. A. Senthilkumar2 and Padmanath Krishnan1*
1Department of Veterinary Biochemistry,
2Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai -51, India
*Corresponding author
Abstract:

Circulating Tumor Cells (CTC’s) serve as a best indicator of underlying cancer. It provides information about the cancer from where they arise and can be used as a tool for diagnosis, treatment and prognosis. The study was conducted with a objective to find a suitable, cost effective technique to capture CTC’s and to develop minimal invasive method for diagnosing tumor/cancer. In the present study polystyrene was used as a matrix to capture circulating tumor cells by using EpCAM antibodies. Tumor cells were spiked in to the canine blood, used for binding assay. FITC tagged cytokeratin was used to label and identify bound tumor cells. We found that tumor cells bound to the antibody coated polystyrene matrix even at low concentrations. Hence this technique can be efficiently be used as a probe to detect cancer.


Keywords: Circulating tumor cells, polystyrene probe, EpCAM, Cytokeratin, Specificity
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How to cite this article:

Karthikeyan Ramaiyan, Pandiyan Velayutham, T. M. A. Senthilkumar and Padmanath Krishnan. 2020. A Probe can Capture Circulating Tumor Cells (CTC) – An Antitumor Antibody based Capture Technique.Int.J.Curr.Microbiol.App.Sci. 9(7): 3748-3755. doi: https://doi.org/10.20546/ijcmas.2020.907.439