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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 3572-3579
DOI: https://doi.org/10.20546/ijcmas.2020.907.417


Sperm Morphology and DNA Integrity of Frozen Thawed Buffalo Semen Treated with Heparin Binding Protein
Yanben M. Kikon1, K. Loganathasamy2*, V. S. Gomathyand K. Vijayarani4
1Department of Veterinary Physiology, Madras Veterinary College, Chennai-600 007, Tamil Nadu, India
2Department of Veterinary Physiology and Biochemistry, Veterinary College and Research Institute, Tirunelveli-627 358, Tamil Nadu, India
3Department of Veterinary Physiology, Madras Veterinary College, Chennai-600 007, Tamil Nadu, India
4Department of Bioinformatics and ARIS Cell, Madras Veterinary College, Chennai-600 007, Tamil Nadu, India
*Corresponding author
Abstract:

The present study was conducted to assess the sperm morphology and DNA integrity of frozen thawed buffalo semen treated with heparin binding protein (HBP). Buffalo semen straws from 10 bulls were procured from Central Frozen Semen Production and Training Institute, Hesseraghatta, Banglore-560088. The frozen straws were thawed at 37ºC for 30 seconds and emptied into a 15mL sterile plastic centrifuge tube containing 1mL capacitation medium (control), addition of 25µg/mL (treatment I), 50µg/mL (treatment II) and 100µg/mL (treatment III) of HBP. The contents were incubated at 37ºC for 2 hours. After incubation, the sperm morphology was determined by Rose Bengal stain technique. Morphologically normal sperm in control, HBP treatment I, II and III were 86.70% ± 1.52, 87.00% ± 1.21, 87.00% ± 1.28 and 86.55% ± 1.59 respectively. There was no significant difference between control and HBP treatments with respect to sperm morphology. The sperm DNA integrity was assessed by acridine orange stain method. There was no significant difference among control (90.15% ± 1.14), HBP treatment I (90.25% ± 1.25), II (89.85% ± 1.05) and III (90.40% ± 1.16) with respect to sperm DNA integrity. This study indicated that HBP supplementation in capacitation medium did not alter the sperm morphology and DNA integrity.


Keywords: Heparin binding protein, Morphology, DNA integrity, Spermatozoa, Buffalo semen
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How to cite this article:

Yanben M. Kikon, K. Loganathasamy, V. S. Gomathy and Vijayarani, K. 2020. Sperm Morphology and DNA Integrity of Frozen Thawed Buffalo Semen Treated with Heparin Binding Protein.Int.J.Curr.Microbiol.App.Sci. 9(7): 3572-3579. doi: https://doi.org/10.20546/ijcmas.2020.907.417