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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 2987-2993
DOI: https://doi.org/10.20546/ijcmas.2020.907.352


Molecular Analysis of Diversity Presents in Brassica juncea genotypes with the Help of SSR Markers
Nupur Saini1* and Archana N. Rai2
1Department of Plant Molecular Biology and Biotechnology, IGKV, Raipur 492012, India
2Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, India
*Corresponding author
Abstract:

Brassica juncea is one of the most important oilseed crops of India, but its genetic diversity is still not explored properly. A better understanding on this topic is a prerequisite for the better utilization of genotypes for breeding programs as well as in crop improvement. This study was undertaken to identify the polymorphism among thirty six genotypes including pure lines/varieties/ accession lines of different agro-climatic areas using fifteen SSR markers. All the thirty six genotypes were raised in pots for extraction of genomic DNA from seven days old seedlings. Amplification of the genomic DNA was carried out using a fifteen primer pairs. Out of the fifteen primers tested, seven reported polymorphism and a total of 32 alleles were amplified. The number of alleles per primer varied from one to three, with an average of 1.5 fragments, while the size of the fragments ranged from 200bp to 400bp. Jaccard’s similarity coefficients based on SSR data ranged from 0.36 to 0. 1. The study focuses on using SSR markers as a stronger and reliable tool for diversity studies.


Keywords: Brassica juncea, SSR markers, Polymorphism and genetic diversity
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How to cite this article:

Nupur Saini and Archana N. Rai. 2020. Molecular Analysis of Diversity Presents in Brassica juncea genotypes with the Help of SSR Markers.Int.J.Curr.Microbiol.App.Sci. 9(7): 2987-2993. doi: https://doi.org/10.20546/ijcmas.2020.907.352