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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 2831-2839
DOI: https://doi.org/10.20546/ijcmas.2020.907.335


Antimicrobial Susceptibility and Resistance Pattern of Mastitis Milk Samples from Chittoor District, Andhra Pradesh, India
D. Rani Prameela1*, D. Sreenivasulu2, N. Mangadevi1 and A. Karthik1
1State Level Diagnostic Laboratory, Sri Venkateswara Veterinary University, Tirupati-517502, Chittoor District, Andhra Pradesh, India
2Director of Extension, SVVU, Tirupati, India
*Corresponding author
Abstract:

Mastitis has been a major problem to the dairy industry and pose main impact on the economy of dairy farmers. Antibiotic resistance is increasing day by day and has become a big challenge worldwide with public health significance. In the present study antimicrobial resistance and sensitivity pattern of mastitis milk samples were assessed during the period from August, 2015 to March, 2020 at State level diagnostic laboratory, Sri Venkateswara Veterinary University, Tirupati. A total of 2,488 milk samples were subjected for cultural isolation and invitro antibiotic sensitivity test with standard disc diffusion method (Bauer et al., 1966). Out of 2,488 milk samples on cultural isolation, 2,174 (87.38%) of gram positive and 314 (12.62%) of gram negative bacteria were identified. Among 2,174 gram positive bacteria, Staphylococcus aureus -2,133(98.11%) was predominant followed by Streptococci-20 (0.92%) and bacillus species -19 (0.87%). Similarly, among 314 gram negative bacteria, E.coli-255(81.20%) were major pathogens followed by Klebsiella-53 (16.88%) and Pseudomonas -14 (4.46%) were identified. Invitro, antibiotic sensitivity test results of gram positive bacterial isolates (2,174) showed resistance and sensitivity to Amikacin (90.4% ; 9.60%) followed by Kanamycin (88.4%;11.6%), Streptomycin (87.5%; 12.50%), Tetracycline (79.6 ; 20.4%), Gentamycin (75.3% ; 24.7%), Pencillin (60.2% ; 39.8%),Ciprofloxacin (52.50 ; 45.7%), Enrofloxacin (48.9%; 51.20), Ampicillin (45.1%;54.9%) and Amoxycillin (36.4% ; 63.6%) respectively. Whereas the isolates of gram negative bacteria revealed resistance and sensitivity to Kanamycin-78.71%; 21.30% followed by Pencillin-76.81%; 23.10%, amikacin-69.11%; 30.90%), Tetracyclin (66.61% ; 33.40%), Streptomycin-(66.6%;33.40%), Ampicillin (64.70%;35.40), Gentamycin (51.0%; 49.0%), Ciprofloxacin (42.0% ; 58.0%) and Enrofloxacin-(34.71%;65.30%) (109/314).


Keywords: Dairy industry-Economy, Antibiotics, Resistance and Sensitivity, Staphylococcus aureus and E.coli
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How to cite this article:

Rani Prameela, D., D. Sreenivasulu, N. Mangadevi and Karthik, A. 2020. Antimicrobial Susceptibility and Resistance Pattern of Mastitis Milk Samples from Chittoor District, Andhra Pradesh, India.Int.J.Curr.Microbiol.App.Sci. 9(7): 2831-2839. doi: https://doi.org/10.20546/ijcmas.2020.907.335