National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Effect of sunlight and UV light on the inactivation of nuclear polyhedrosis virus to Diaphania pulverulentalis (Hampson). Larvae were studied under laboratory conditions. The larval mortality was significantly maximum (76.66%) and (86.65%) when DpNPV suspension and formulation was exposed to sunlight for 1 hour respectively. Minimum mortality of (48.33%) and (61.66%) of DpNPV suspension and formulation were observed when it was exposed to sunlight for 5 hours which indicating reverse relationship between the exposure duration to sunlight and larval mortality. Inactivation of DpNPV by UV light was demonstrated by the decreased mortality rate in larval groups inoculated with irradiated virus compared to those fed on non- radiated virus i,e., control. Significant difference in larval mortality was observed when DpNPV was exposed to 5, 10, 15, 30 and 60 minutes to UV light, (88.33, 85.00, 80.00, 66.66 and 53.33 % mortality respectively). Whereas in formulated DpNPV, the rate of larval mortality recorded was 93.33, 90.00, 86.65, 71.66 and 66.66 per cent when exposed to 5, 10, 15, 30 and 60 minutes to UV light respectively. The inactivation of virus was directly related to the period of exposure to UV radiation. This finding indicated a loss in pathogenicity and the number of days to death was higher and the total mortality was lower from exposed virus than from unexposed virus.
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