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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 2776-2784
DOI: https://doi.org/10.20546/ijcmas.2020.907.327


Indirect Organogenesis and Plant Regeneration in Pointed Gourd (Trichosanthes dioica Roxb.), an Important Perennial Vegetable
Manoranjan Prusty1*, Kailash Chandra Samal2, Gouri Shankar Sahu2, Tusar Ranjan Sahoo3, Laxmipriya Sahoo1 and Tania Seth1
1ICAR-Central Institute for Women in Agriculture, Bhubaneswar - 751003, Odisha, India
2Odisha University of Agriculture & Technology, Bhubaneswar - 751003, Odisha, India
3KVK, ICAR-National Rice Research Institute, Cuttack - 753006, Odisha, India
*Corresponding author
Abstract:

Pointed gourd (Trichosanthes dioica Roxb.) is one of the important nutritive cucurbit perennial vegetables in India and other tropical countries. In the present investigation, an efficient and reproducible protocol for clonal multiplication through in vitro culture of Pointed gourd (Trichosanthes dioica Roxb.) is standardized. High frequency organogenic callusing from shoot-tip and nodal segments was induced by culturing the explants on MS medium supplemented with 4µM 2-4-D. The maximum regeneration of shoots from callus (72.47 %) and highest callus weight per explant (0.5 g) was recorded in variety ‘Nayagarh Local (Female)’. The highest number of shoots (3.0) per callus was regenerated from variety ‘Swarna Aloukik (Female)’ when cultured in MS medium fortified with BAP (4 µM). The rooted plantlets were transferred to soil mixture (soil: sand: cocopit; 1:1:1) and kept in greenhouse with 85% humidity. After 2-3 weeks hardening, the plantlets were successfully transplanted in the polybag or in the field with higher survival rate (85%). No detectable variation in the regenerated plants with respect to morphology or growth characteristics was detected in the field as compared to the mother plant.These results may be great helpful for production of number of quality, uniform, disease free and desired plantlets for distribution to the farmers.


Keywords: Pointed Gourd (Trichosanthes dioica), Indirect Organogenesis, Plant Regeneration
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How to cite this article:

Manoranjan Prusty, Kailash Chandra Samal, Gouri Shankar Sahu, Tusar Ranjan Sahoo Laxmipriya Sahoo and Tania Seth. 2020. Indirect Organogenesis and Plant Regeneration in Pointed Gourd (Trichosanthes dioica Roxb.), an Important Perennial Vegetable.Int.J.Curr.Microbiol.App.Sci. 9(7): 2776-2784. doi: https://doi.org/10.20546/ijcmas.2020.907.327