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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 2534-2541
DOI: https://doi.org/10.20546/ijcmas.2020.907.297


Production and Standardization of Combined Vaccine against Haemorrhagic Septicaemia and Black Quarter
Nivedita Kushram1, Rakesh Sharda2, Kripa Shankar Misraulia1, Sanjay Madhukar Gholap3, Daljeet Chhabra2, Hemant Mehta2 and Rakhi Gangil2*
1Department of Veterinary Medicine, NDVSU
2Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Mhow -453446, India
3Institute of Animal Health and Veterinary Biologicals, Mhow -453446, India
*Corresponding author
Abstract:

The enormosity of infectious diseases continues to be a major constraint to the economical viability of livestock industry. Combined vaccines against more than one infectious disease reduce the cost, time, and labour of vaccination. In present study, formalized vaccines against Haemorrhagic Septicaemia and Black Quarter diseases were prepared using P52 strain of Pasteurella multocida and Clostridium chauvoei strain 49. Different proportions of these two vaccines were mixed to formulate various groups of combined vaccines, which were subsequently standardized for sterility, safety and potency. Amongst all combinations a group containing 1 ml of H.S. casein sucrose yeast extract agar washed vaccine and 4 ml of B.Q. thioglycolate broth and culture vaccine with shaking was found most efficacious in our study. This combined vaccine conferred 100% protection against H.S. disease and 75% against B.Q. disease in rabbits and guinea pigs, respectively in potency test performed for individual vaccines. The study also revealed that casein sucrose yeast agar and fluid thioglycolate broth are better medium for cultivation of P. multocida and Cl. chauvoei. Enhanced growth of Cl. chauvoei was observed when incubated on a shaker as compared to static culture.


Keywords: Combined vaccine, Clostridium chauvoei, Black quarter, Haemorrhagic septicaemia, Pasteurella multocida
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How to cite this article:

Nivedita Kushram, Rakesh Sharda, Kripa Shankar Misraulia, Sanjay Madhukar Gholap, Daljeet Chhabra, Hemant Mehta and Rakhi Gangil. 2020. Production and Standardization of Combined Vaccine against Haemorrhagic Septicaemia and Black Quarter.Int.J.Curr.Microbiol.App.Sci. 9(7): 2534-2541. doi: https://doi.org/10.20546/ijcmas.2020.907.297