Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 2517-2526
DOI: https://doi.org/10.20546/ijcmas.2020.907.295


Indirect Organogenesis for Regeneration in Brinjal (Solanum melongena L.) Var. KKM 1
A. Nivetha1, S. Merina PremKumari2*, R. Kannan1, R. Arulmozhiyan3, N. Rajinimala1 and M. Arumugam Pillai2
1Department of Plant Pathology, Agricultural College and Research Institute, Killikulam, Vallanadu-628252, Tamil Nadu, India
2Biotechnology Division, Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Killikulam, Vallanad-628252, Tamil Nadu, India
3Department of Horticulture, Agricultural College and Research Institute, Killikulam, Vallanadu-628252, Tamil Nadu, India
*Corresponding author
Abstract:

Brinjal is an important nutritional vegetable crop of tropical and subtropical regions. It is highly responsive to various tissue culture techniques. The aim of this study was to develop an efficient protocol to standardize the concentration of sterilants for seed and explants sterilization for in vitro germination and regeneration respectively. The explants used are cotyledonary leaf, stem and hypocotyl of in vitro grown brinjal var.KKM1. For sterilization of seeds and explants, 5% sodium hypochlorite treatment for 3 minutes is the most effective sterilant concentration against the microbiological contamination. In subsequence, the regeneration protocol was optimised for the brinjal var.KKM1. From the in vitro grown seedlings, different explants such as hypocotyl, cotyledenary leaf and stem were taken for callus induction. Highest number of callus was obtained in MS medium containing 2 mgl-1 NAA and 0.5 mgl-1 BAP. Among the explants used, cotyledonary leaf and stem were highly responsive to callus induction. Further the callus was proliferated in the same media and was used for regeneration by using different plant growth hormones. Regeneration of shoots from callus was more efficient in MS media containing 2.0 mgl-1 BAP, 1.0 mgl-1 Kn and 0.5 mgl-1 NAA. The successful rooting was recorded on ½ MS media supplemented with 0.5 mgl-1 IBA. This in vitro regeneration process might be useful for producing disease free plants especially little leaf of brinjal, the most common infection in the brinjal var.KKM1.


Keywords: Brinjal variety KKM1 seeds, Explants, Sterilization, Callus induction, Regeneration
Download this article as Download

How to cite this article:

Nivetha, A., S. Merina Prem Kumari, R. Kannan, R. Arulmozhiyan, N. Rajinimala and Arumugam Pillai, M. 2020. Indirect Organogenesis for Regeneration in Brinjal (Solanum melongena L.) Var. KKM 1.Int.J.Curr.Microbiol.App.Sci. 9(7): 2517-2526. doi: https://doi.org/10.20546/ijcmas.2020.907.295