National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The aim of present study was to assess the genetic diversity, genetic distance and establish genetic relationship between Ongole and Punganur zebu cattle breeds using fifteen bovine specific dinucleotide markers. A total of 78 DNA samples from unrelated Ongole and Punganur cattle breeds were genotyped to find out within and between breed genetic diversity indices. The mean number of alleles (7.67); effective number of alleles (4.53); observed heterozygosity (0.31) and expected heterozygosity (0.74) reflected the existence of substantial within breed diversity in both the cattle breeds. The mean estimates of FIS, FIT and FST were 0.47, 0.57 and 0.18 respectively. The Nei’s standard and D-genetic estimates revealed high genetic distance (1.1152) between two breeds and less genetic identity (0.3279) among the breeds. UPGMA method was used to construct dendrogram and to find out genetic distance between two breeds. The length between population one and two was found to be 55.7596. The mean value of Shannon index is 1.69, which clearly indicates high gene diversity within populations. Ewens-Watterson test was conducted to test the neutrality of microsatellites. The rate of gene flow between Ongole and Punganur cattle was found to be 1.11.
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