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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 1650-1654
DOI: https://doi.org/10.20546/ijcmas.2017.603.191


Genetic Diversity and Phylogenetic Relationship between Indigenous Cattle Breeds
S. Vani* and K. Sakunthala Devi
Department of Animal Genetics and Breeding College of Veterinary Science, Proddatur, Y.S.R District, A.P – 516360, India
*Corresponding author
Abstract:

The aim of present study was to assess the genetic diversity, genetic distance and establish genetic relationship between Ongole and Punganur zebu cattle breeds using fifteen bovine specific dinucleotide markers. A total of 78 DNA samples from unrelated Ongole and Punganur cattle breeds were genotyped to find out within and between breed genetic diversity indices. The mean number of alleles (7.67); effective number of alleles (4.53); observed heterozygosity (0.31) and expected heterozygosity (0.74) reflected the existence of substantial within breed diversity in both the cattle breeds. The mean estimates of FIS, FIT and FST were 0.47, 0.57 and 0.18 respectively. The Nei’s standard and D-genetic estimates revealed high genetic distance (1.1152) between two breeds and less genetic identity (0.3279) among the breeds. UPGMA method was used to construct dendrogram and to find out genetic distance between two breeds. The length between population one and two was found to be 55.7596. The mean value of Shannon index is 1.69, which clearly indicates high gene diversity within populations. Ewens-Watterson test was conducted to test the neutrality of microsatellites. The rate of gene flow between Ongole and Punganur cattle was found to be 1.11.


Keywords: Genetic relationship, Genetic distance, Phylogenetic analysis, Microsatellite markers, Zebu cattle.
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How to cite this article:

Vani, S. and Sakunthala Devi, K. 2017. Genetic Diversity and Phylogenetic Relationship between Indigenous Cattle BreedsInt.J.Curr.Microbiol.App.Sci. 6(3): 1650-1654. doi: https://doi.org/10.20546/ijcmas.2017.603.191