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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 2358-2364
DOI: https://doi.org/10.20546/ijcmas.2020.907.275


Molecular Characterisation of DNA of Brown Plant Hopper Biotypes (Nilaparvata lugens L.) Under Screening Study of Brown Plant Hopper Resistance in Various Rice Genotypes at Gangavathi
Farzana Korabu*, B. Kisan, I. Shanker Goud, Ayyangowda Patil, Mahantesh Shivyoggaya and Sujay Hurali
Department of Genetics and Plant Breeding, College of Agriculture, Raichur University of Agricultural Sciences, Raichur-584101, Karnataka, India
*Corresponding author
Abstract:

The Brown plant Hopper (BPH) Nilaparvata lugens L. (Homoptera: Delphacidae) is one of the most destructive insect pests causing significant yield loss in most of the rice cultivars of India as well as Asia.it is a typical phloem feeder. Hence, present study was undertaken on DNA molecular characterization of BPH population using SSR markers that will give clear understanding of BPH diversity in Gangavathi region. Ten SSR markers were screened in the selected 25 BPHs and all ten markers were screened to assess the molecular diversity.Genotyping data of SSR markers were analyzed using Unweight pair group method with Arithmetic Mean (UPGMA) method using DARwin V6.0. This analysis clustered 25 BPH populations into three groups. Cluster-I had highest number of BPH possessing 21 BPH population. Cluster-II with three BPH and cluster-III had one solitary BPH population. This indicates that majority of the BPH populations are similar at Gangavathi whereas two biotypes are quite different.


Keywords: BPH, Primers, SSR markers
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How to cite this article:

Farzana Korabu, B. Kisan, I. Shanker Goud, Ayyangowda Patil, Mahantesh Shivyoggaya and Sujay Hurali. 2020. Molecular Characterisation of DNA of Brown Plant Hopper Biotypes (Nilaparvata lugens L.) Under Screening Study of Brown Plant Hopper Resistance in Various Rice Genotypes at Gangavathi.Int.J.Curr.Microbiol.App.Sci. 9(7): 2358-2364. doi: https://doi.org/10.20546/ijcmas.2020.907.275