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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 2273-2286
DOI: https://doi.org/10.20546/ijcmas.2020.907.265


Culture Establishment of Coccinia grandis (L.) through Nodal Segments under in vitro Conditions
Mohammad Amin1*, A. V. D. Dorajee Rao2, K. Ravindra Kumar3 and D. R. Salomi Suneetha4
1Department of Vegetable Science
2 ,3Department of Horticulture
4Department of Biochemistry, College of Horticulture, Dr. Y. S. R Horticultural University, Venkataramannagudem, West Godavari, India
*Corresponding author
Abstract:

An experiment was conducted on culture establishment of Coccinia grandis (L.) through nodal segments under in vitro conditions with an objective to standardise a viable protocol for in vitro propagation. Significant results were observed for number of buds sprouted, percent of shoot bud induction, number of days taken for shoot initiation, number of shoots per explant, shoot length, number of leaves per shoot and vitrification For shoot induction and succeeding growth was effectively induced by TDZ at lower (0.5 mg l-1) concentration as compared to higher (1.0 mg l-1) concentration). For shoot proliferation 3.0 mg l-1 BAP + 0.25 mg l-1 NAA (T4) and 4.0 mg l-1 BAP + 0.25 mg l-1 NAA (T5) in MS medium were more efficient for inducing maximum number of micro shoots and leaves.


Keywords: Coccinia, Nodal segments, Culture establishment, Shoot proliferation, In vitro
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How to cite this article:

Mohammad Amin, A. V. D. Dorajee Rao, K. Ravindra Kumar and Salomi Suneetha, D. R. 2020. Culture Establishment of Coccinia grandis (L.) Through Nodal Segments under in vitro Conditions.Int.J.Curr.Microbiol.App.Sci. 9(7): 2273-2286. doi: https://doi.org/10.20546/ijcmas.2020.907.265